Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

John S. Reece-Hoyes, A. Rasim Barutcu, Rachel Patton McCord, Jun Seop Jeong, Lizhi Jiang, Andrew MacWilliams, Xinping Yang, Kourosh Salehi-Ashtiani, David E. Hill, Seth Blackshaw, Heng Zhu, Job Dekker, Albertha J.M. Walhout

    Research output: Contribution to journalArticle

    Abstract

    Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

    Original languageEnglish (US)
    Pages (from-to)1050-1054
    Number of pages5
    JournalNature Methods
    Volume8
    Issue number12
    DOIs
    StatePublished - Dec 1 2011

    Fingerprint

    Gene Regulatory Networks
    Yeast
    Assays
    Transcription Factors
    Genes
    Yeasts
    DNA sequences
    DNA
    Clone Cells
    Proteins

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry
    • Molecular Biology
    • Cell Biology

    Cite this

    Reece-Hoyes, J. S., Barutcu, A. R., McCord, R. P., Jeong, J. S., Jiang, L., MacWilliams, A., ... Walhout, A. J. M. (2011). Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nature Methods, 8(12), 1050-1054. https://doi.org/10.1038/nmeth.1764

    Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. / Reece-Hoyes, John S.; Barutcu, A. Rasim; McCord, Rachel Patton; Jeong, Jun Seop; Jiang, Lizhi; MacWilliams, Andrew; Yang, Xinping; Salehi-Ashtiani, Kourosh; Hill, David E.; Blackshaw, Seth; Zhu, Heng; Dekker, Job; Walhout, Albertha J.M.

    In: Nature Methods, Vol. 8, No. 12, 01.12.2011, p. 1050-1054.

    Research output: Contribution to journalArticle

    Reece-Hoyes, JS, Barutcu, AR, McCord, RP, Jeong, JS, Jiang, L, MacWilliams, A, Yang, X, Salehi-Ashtiani, K, Hill, DE, Blackshaw, S, Zhu, H, Dekker, J & Walhout, AJM 2011, 'Yeast one-hybrid assays for gene-centered human gene regulatory network mapping', Nature Methods, vol. 8, no. 12, pp. 1050-1054. https://doi.org/10.1038/nmeth.1764
    Reece-Hoyes JS, Barutcu AR, McCord RP, Jeong JS, Jiang L, MacWilliams A et al. Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nature Methods. 2011 Dec 1;8(12):1050-1054. https://doi.org/10.1038/nmeth.1764
    Reece-Hoyes, John S. ; Barutcu, A. Rasim ; McCord, Rachel Patton ; Jeong, Jun Seop ; Jiang, Lizhi ; MacWilliams, Andrew ; Yang, Xinping ; Salehi-Ashtiani, Kourosh ; Hill, David E. ; Blackshaw, Seth ; Zhu, Heng ; Dekker, Job ; Walhout, Albertha J.M. / Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. In: Nature Methods. 2011 ; Vol. 8, No. 12. pp. 1050-1054.
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