Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

John S. Reece-Hoyes, A. Rasim Barutcu, Rachel Patton McCord, Jun Seop Jeong, Lizhi Jiang, Andrew MacWilliams, Xinping Yang, Kourosh Salehi-Ashtiani, David E. Hill, Seth Blackshaw, Heng Zhu, Job Dekker, Albertha J.M. Walhout

Research output: Contribution to journalArticle

Abstract

Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

Original languageEnglish (US)
Pages (from-to)1050-1054
Number of pages5
JournalNature Methods
Volume8
Issue number12
DOIs
StatePublished - Dec 1 2011

Fingerprint

Gene Regulatory Networks
Yeast
Assays
Transcription Factors
Genes
Yeasts
DNA sequences
DNA
Clone Cells
Proteins

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Reece-Hoyes, J. S., Barutcu, A. R., McCord, R. P., Jeong, J. S., Jiang, L., MacWilliams, A., ... Walhout, A. J. M. (2011). Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nature Methods, 8(12), 1050-1054. https://doi.org/10.1038/nmeth.1764

Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. / Reece-Hoyes, John S.; Barutcu, A. Rasim; McCord, Rachel Patton; Jeong, Jun Seop; Jiang, Lizhi; MacWilliams, Andrew; Yang, Xinping; Salehi-Ashtiani, Kourosh; Hill, David E.; Blackshaw, Seth; Zhu, Heng; Dekker, Job; Walhout, Albertha J.M.

In: Nature Methods, Vol. 8, No. 12, 01.12.2011, p. 1050-1054.

Research output: Contribution to journalArticle

Reece-Hoyes, JS, Barutcu, AR, McCord, RP, Jeong, JS, Jiang, L, MacWilliams, A, Yang, X, Salehi-Ashtiani, K, Hill, DE, Blackshaw, S, Zhu, H, Dekker, J & Walhout, AJM 2011, 'Yeast one-hybrid assays for gene-centered human gene regulatory network mapping', Nature Methods, vol. 8, no. 12, pp. 1050-1054. https://doi.org/10.1038/nmeth.1764
Reece-Hoyes JS, Barutcu AR, McCord RP, Jeong JS, Jiang L, MacWilliams A et al. Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. Nature Methods. 2011 Dec 1;8(12):1050-1054. https://doi.org/10.1038/nmeth.1764
Reece-Hoyes, John S. ; Barutcu, A. Rasim ; McCord, Rachel Patton ; Jeong, Jun Seop ; Jiang, Lizhi ; MacWilliams, Andrew ; Yang, Xinping ; Salehi-Ashtiani, Kourosh ; Hill, David E. ; Blackshaw, Seth ; Zhu, Heng ; Dekker, Job ; Walhout, Albertha J.M. / Yeast one-hybrid assays for gene-centered human gene regulatory network mapping. In: Nature Methods. 2011 ; Vol. 8, No. 12. pp. 1050-1054.
@article{1f6c4d039b87426c89d7f0536053e122,
title = "Yeast one-hybrid assays for gene-centered human gene regulatory network mapping",
abstract = "Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69{\%}) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.",
author = "Reece-Hoyes, {John S.} and Barutcu, {A. Rasim} and McCord, {Rachel Patton} and Jeong, {Jun Seop} and Lizhi Jiang and Andrew MacWilliams and Xinping Yang and Kourosh Salehi-Ashtiani and Hill, {David E.} and Seth Blackshaw and Heng Zhu and Job Dekker and Walhout, {Albertha J.M.}",
year = "2011",
month = "12",
day = "1",
doi = "10.1038/nmeth.1764",
language = "English (US)",
volume = "8",
pages = "1050--1054",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Public Library of Science",
number = "12",

}

TY - JOUR

T1 - Yeast one-hybrid assays for gene-centered human gene regulatory network mapping

AU - Reece-Hoyes, John S.

AU - Barutcu, A. Rasim

AU - McCord, Rachel Patton

AU - Jeong, Jun Seop

AU - Jiang, Lizhi

AU - MacWilliams, Andrew

AU - Yang, Xinping

AU - Salehi-Ashtiani, Kourosh

AU - Hill, David E.

AU - Blackshaw, Seth

AU - Zhu, Heng

AU - Dekker, Job

AU - Walhout, Albertha J.M.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

AB - Gateway-compatible yeast one-hybrid (Y1H) assays provide a convenient gene-centered (DNA to protein) approach to identify transcription factors that can bind a DNA sequence of interest. We present Y1H resources, including clones for 988 of 1,434 (69%) predicted human transcription factors, that can be used to detect both known and new interactions between human DNA regions and transcription factors.

UR - http://www.scopus.com/inward/record.url?scp=82355190165&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=82355190165&partnerID=8YFLogxK

U2 - 10.1038/nmeth.1764

DO - 10.1038/nmeth.1764

M3 - Article

VL - 8

SP - 1050

EP - 1054

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 12

ER -