X-gal staining of electroporated sea squirt (Ciona) embryos

Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine

Research output: Contribution to journalArticle

Abstract

This protocol describes the fixation, staining, and mounting of sea squirt (Ciona intestinalis) embryos and larvae that have been electroporated with a plasmid DNA containing a cis-regulatory DNA (e.g., tissue-specific enhancer) fused to the lacZ reporter gene. Green fluorescent protein (GFP) reporter genes can be directly visualized in living embryos and larvae, although fixed preparations can use aspects of this protocol.

Original languageEnglish (US)
JournalCold Spring Harbor Protocols
Volume4
Issue number12
DOIs
StatePublished - 2009

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Reporter Genes
Oceans and Seas
Larva
Embryonic Structures
Genes
Ciona intestinalis
Staining and Labeling
Lac Operon
DNA
Green Fluorescent Proteins
Mountings
Plasmids
Tissue
5-bromo-4-chloro-3-indolyl beta-galactoside

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

X-gal staining of electroporated sea squirt (Ciona) embryos. / Christiaen, Lionel; Wagner, Eileen; Shi, Weiyang; Levine, Michael.

In: Cold Spring Harbor Protocols, Vol. 4, No. 12, 2009.

Research output: Contribution to journalArticle

Christiaen, Lionel ; Wagner, Eileen ; Shi, Weiyang ; Levine, Michael. / X-gal staining of electroporated sea squirt (Ciona) embryos. In: Cold Spring Harbor Protocols. 2009 ; Vol. 4, No. 12.
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