X chromosome repression by localization of the C. elegans dosage compensation machinery to sites of transcription initiation

Sevinc Ercan, Paul G. Giresi, Christina M. Whittle, Xinmin Zhang, Roland D. Green, Jason D. Lieb

Research output: Contribution to journalArticle

Abstract

Among organisms with chromosome-based mechanisms of sex determination, failure to equalize expression of X-linked genes between the sexes is typically lethal. In C. elegans, XX hermaphrodites halve transcription from each X chromosome to match the output of XO males. Here, we mapped the binding location of the condensin homolog DPY-27 and the zinc finger protein SDC-3, two components of the C. elegans dosage compensation complex (DCC). We observed strong foci of DCC binding on X, surrounded by broader regions of localization. Binding foci, but not adjacent regions of localization, were distinguished by clusters of a 10-bp DNA motif, suggesting a recruitment-and-spreading mechanism for X recognition. The DCC was preferentially bound upstream of genes, suggesting modulation of transcriptional initiation and polymerase-coupled spreading. Stronger DCC binding upstream of genes with high transcriptional activity indicated a mechanism for tuning DCC activity at specific loci. These data aid in understanding how proteins involved in higher-order chromosome dynamics can regulate transcription at individual loci.

Original languageEnglish (US)
Pages (from-to)403-408
Number of pages6
JournalNature Genetics
Volume39
Issue number3
DOIs
StatePublished - Mar 2007

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Transcription Initiation Site
X Chromosome
Chromosomes
X-Linked Genes
Nucleotide Motifs
Zinc Fingers
Genes
Proteins
condensin complexes

ASJC Scopus subject areas

  • Genetics(clinical)
  • Genetics

Cite this

X chromosome repression by localization of the C. elegans dosage compensation machinery to sites of transcription initiation. / Ercan, Sevinc; Giresi, Paul G.; Whittle, Christina M.; Zhang, Xinmin; Green, Roland D.; Lieb, Jason D.

In: Nature Genetics, Vol. 39, No. 3, 03.2007, p. 403-408.

Research output: Contribution to journalArticle

Ercan, Sevinc ; Giresi, Paul G. ; Whittle, Christina M. ; Zhang, Xinmin ; Green, Roland D. ; Lieb, Jason D. / X chromosome repression by localization of the C. elegans dosage compensation machinery to sites of transcription initiation. In: Nature Genetics. 2007 ; Vol. 39, No. 3. pp. 403-408.
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