Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos

Lionel Christiaen, Eileen Wagner, Weiyang Shi, Michael Levine

Research output: Contribution to journalArticle

Abstract

This protocol describes whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. This method is a mainstay of the sea squirt (Ciona) research community. It permits the detailed visualization of gene expression at single-cell resolution. It has been used to detect localized maternal mRNAs in the fertilized egg and to identify restricted patterns of gene expression within individual cells of the developing central nervous system at advanced stages of development, including swimming tadpoles.

Original languageEnglish (US)
JournalCold Spring Harbor Protocols
Volume4
Issue number12
DOIs
StatePublished - 2009

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Ciona intestinalis
Gene expression
Oceans and Seas
In Situ Hybridization
Embryonic Structures
Stored Messenger RNA
Gene Expression
Zygote
Neurology
Larva
Visualization
Central Nervous System
Research

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. / Christiaen, Lionel; Wagner, Eileen; Shi, Weiyang; Levine, Michael.

In: Cold Spring Harbor Protocols, Vol. 4, No. 12, 2009.

Research output: Contribution to journalArticle

Christiaen, Lionel ; Wagner, Eileen ; Shi, Weiyang ; Levine, Michael. / Whole-mount in situ hybridization on sea squirt (Ciona intestinalis) embryos. In: Cold Spring Harbor Protocols. 2009 ; Vol. 4, No. 12.
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