Week-long imaging of cell divisions in the Arabidopsis root meristem

Ramin Rahni, Kenneth Birnbaum

Research output: Contribution to journalArticle

Abstract

Background: Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week. Results: Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. Conclusions: These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning.

Original languageEnglish (US)
Article number30
JournalPlant Methods
Volume15
Issue number1
DOIs
StatePublished - Mar 25 2019

Fingerprint

Meristem
root meristems
Arabidopsis
Cell Division
cell division
image analysis
Stem Cell Niche
stem cells
niches
direct contact
Confocal Microscopy
Stem Cells
Arabidopsis thaliana
cells
methodology

Keywords

  • Cell division
  • Confocal
  • Development
  • Live imaging
  • QC
  • Root
  • Stem cells
  • Time lapse

ASJC Scopus subject areas

  • Biotechnology
  • Genetics
  • Plant Science

Cite this

Week-long imaging of cell divisions in the Arabidopsis root meristem. / Rahni, Ramin; Birnbaum, Kenneth.

In: Plant Methods, Vol. 15, No. 1, 30, 25.03.2019.

Research output: Contribution to journalArticle

@article{0b1d36e1166d4c588859a44fcf8fdfb5,
title = "Week-long imaging of cell divisions in the Arabidopsis root meristem",
abstract = "Background: Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week. Results: Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. Conclusions: These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning.",
keywords = "Cell division, Confocal, Development, Live imaging, QC, Root, Stem cells, Time lapse",
author = "Ramin Rahni and Kenneth Birnbaum",
year = "2019",
month = "3",
day = "25",
doi = "10.1186/s13007-019-0417-9",
language = "English (US)",
volume = "15",
journal = "Plant Methods",
issn = "1746-4811",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Week-long imaging of cell divisions in the Arabidopsis root meristem

AU - Rahni, Ramin

AU - Birnbaum, Kenneth

PY - 2019/3/25

Y1 - 2019/3/25

N2 - Background: Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week. Results: Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. Conclusions: These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning.

AB - Background: Characterizing the behaviors of dynamic systems requires capturing them with high temporal and spatial resolution. Owing to its transparency and genetic tractability, the Arabidopsis thaliana root lends itself well to live imaging when combined with cell and tissue-specific fluorescent reporters. We developed a novel 4D imaging method that utilizes simple confocal microscopy and readily available components to track cell divisions in the root stem cell niche and surrounding region for up to 1 week. Results: Using this method, we performed a direct measurement of cell division intervals within and around the root stem cell niche. The results reveal a short, steep gradient of cell division rates in proximal stem cells, with progressively more rapid cell division rates from quiescent center (QC), to cells in direct contact with the QC (initials), to their immediate daughters, after which division rates appear to become more homogeneous. Conclusions: These results provide a baseline to study how perturbations in signaling could affect cell division patterns in the root meristem. This new setup further allows us to finely analyze meristematic cell division rates that lead to patterning.

KW - Cell division

KW - Confocal

KW - Development

KW - Live imaging

KW - QC

KW - Root

KW - Stem cells

KW - Time lapse

UR - http://www.scopus.com/inward/record.url?scp=85063438440&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063438440&partnerID=8YFLogxK

U2 - 10.1186/s13007-019-0417-9

DO - 10.1186/s13007-019-0417-9

M3 - Article

VL - 15

JO - Plant Methods

JF - Plant Methods

SN - 1746-4811

IS - 1

M1 - 30

ER -