Vibrational spectra of chemical and isotopic variants of oxyluciferin, the light emitter of firefly bioluminescence

Oleg V. Maltsev, Ling Yue, Mateusz Rebarz, Lukas Hintermann, Michel Sliwa, Cyril Ruckebusch, Ljupčo Pejov, Ya Jun Liu, Pance Naumov

Research output: Contribution to journalArticle

Abstract

The chemical complexity of oxyluciferin (OxyLH2), the light-emitting molecule in the bioluminescence of fireflies, originates from the possibility of keto/enol tautomerism and single or double deprotonation. Herein, we present detailed infrared spectroscopic analysis of OxyLH2 and several of its chemical isomers and isotopomers. To facilitate the future characterization of its biogenic forms, we provide accurate assignments of the solid-state and solution FTIR spectra of OxyLH2 based on comparison to six isotopically labeled variants ([2-13C]-OxyLH2, [3-15N]-OxyLH2, [4-13C]-OxyLH2, [5-13C]-OxyLH2, [2′-13C]-OxyLH 2, [3′-15N]-OxyLH2), five closely related structural analogues, and theoretically computed spectra. The computed DFT harmonic vibrational force fields (B3LYP and M06 functionals with basis sets of varying flexibility up to 6-311++G) reproduce well the observed shifts in the IR spectra of both isotopically labeled and structurally related analogues. Illuminating IR spectra: A detailed IR spectroscopic study of oxyluciferin, the light emitter of firefly bioluminescence, has been performed, which reveals the most accurate vibrational assignments to date (see figure). To that end, a set of oxyluciferin analogues and isotopomers were prepared, and their spectra were recorded in the solid state and in acetonitrile solution.

Original languageEnglish (US)
Pages (from-to)10782-10790
Number of pages9
JournalChemistry - A European Journal
Volume20
Issue number34
DOIs
StatePublished - Aug 18 2014

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Bioluminescence
Vibrational spectra
Deprotonation
Spectroscopic analysis
Acetonitrile
Discrete Fourier transforms
Isomers
Infrared radiation
Molecules
oxyluciferin

Keywords

  • bioluminescence
  • DFT calculations
  • IR spectroscopy
  • isotope labeling
  • oxyluciferin

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Vibrational spectra of chemical and isotopic variants of oxyluciferin, the light emitter of firefly bioluminescence. / Maltsev, Oleg V.; Yue, Ling; Rebarz, Mateusz; Hintermann, Lukas; Sliwa, Michel; Ruckebusch, Cyril; Pejov, Ljupčo; Liu, Ya Jun; Naumov, Pance.

In: Chemistry - A European Journal, Vol. 20, No. 34, 18.08.2014, p. 10782-10790.

Research output: Contribution to journalArticle

Maltsev, OV, Yue, L, Rebarz, M, Hintermann, L, Sliwa, M, Ruckebusch, C, Pejov, L, Liu, YJ & Naumov, P 2014, 'Vibrational spectra of chemical and isotopic variants of oxyluciferin, the light emitter of firefly bioluminescence', Chemistry - A European Journal, vol. 20, no. 34, pp. 10782-10790. https://doi.org/10.1002/chem.201400210
Maltsev, Oleg V. ; Yue, Ling ; Rebarz, Mateusz ; Hintermann, Lukas ; Sliwa, Michel ; Ruckebusch, Cyril ; Pejov, Ljupčo ; Liu, Ya Jun ; Naumov, Pance. / Vibrational spectra of chemical and isotopic variants of oxyluciferin, the light emitter of firefly bioluminescence. In: Chemistry - A European Journal. 2014 ; Vol. 20, No. 34. pp. 10782-10790.
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AU - Hintermann, Lukas

AU - Sliwa, Michel

AU - Ruckebusch, Cyril

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AB - The chemical complexity of oxyluciferin (OxyLH2), the light-emitting molecule in the bioluminescence of fireflies, originates from the possibility of keto/enol tautomerism and single or double deprotonation. Herein, we present detailed infrared spectroscopic analysis of OxyLH2 and several of its chemical isomers and isotopomers. To facilitate the future characterization of its biogenic forms, we provide accurate assignments of the solid-state and solution FTIR spectra of OxyLH2 based on comparison to six isotopically labeled variants ([2-13C]-OxyLH2, [3-15N]-OxyLH2, [4-13C]-OxyLH2, [5-13C]-OxyLH2, [2′-13C]-OxyLH 2, [3′-15N]-OxyLH2), five closely related structural analogues, and theoretically computed spectra. The computed DFT harmonic vibrational force fields (B3LYP and M06 functionals with basis sets of varying flexibility up to 6-311++G) reproduce well the observed shifts in the IR spectra of both isotopically labeled and structurally related analogues. Illuminating IR spectra: A detailed IR spectroscopic study of oxyluciferin, the light emitter of firefly bioluminescence, has been performed, which reveals the most accurate vibrational assignments to date (see figure). To that end, a set of oxyluciferin analogues and isotopomers were prepared, and their spectra were recorded in the solid state and in acetonitrile solution.

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