Vertical versus planar neural induction in Rana pipiens embryos

Research output: Contribution to journalArticle

Abstract

The neural plate in the amphibian embryo is induced in the ectoderm by signals from the dorsal mesoderm. In the extensively studied species Xenopus laevis, such signals are believed to proceed along two alternate pathways, defined as vertical and planar induction. We have studied the relative importance of these pathways in Rana pipiens. In the embryo of this frog, dorsal mesoderm involution can be diverted from its normal course by injection of peptides that inhibit interaction of fibronectin with its receptor. In such embryos, dorsal mesoderm failed to migrate across the blastocoel roof but moved bilaterally along the equator, leading to the formation of two notochords. Neural tissue differentiation occurred in close association with each notochord, but no neural tissue formed along the dorsal midline as might have been predicted by a predominantly planar induction model. While in X. laevis planar induction has been reported to be a major pathway in neutralizing the ectoderm, the results presented here indicate that vertical induction predominates in initiating neural development in R. pipiens embryos.

Original languageEnglish (US)
Pages (from-to)3049-3053
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number8
StatePublished - Apr 12 1994

Fingerprint

Rana pipiens
Mesoderm
Embryonic Structures
Notochord
Ectoderm
Xenopus laevis
Neural Plate
Amphibians
Fibronectins
Anura
Peptides
Injections

Keywords

  • amphibian
  • gastrulation
  • neural induction

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Vertical versus planar neural induction in Rana pipiens embryos. / Saint-Jeannet, Jean-Pierre; Dawid, Igor B.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 8, 12.04.1994, p. 3049-3053.

Research output: Contribution to journalArticle

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