Vertical movement in soil of insecticidal Cry1Ab protein from Bacillus thuringiensis

Deepak Saxena, Saul Flores, G. Stotzky

Research output: Contribution to journalArticle

Abstract

The effects of montmorillonite (M) or kaolinite (K) on the vertical movement of the insecticidal Cry1Ab protein of Bacillus thuringiensis subsp. kurstaki (Bt) were studied in repacked soil columns. The protein was added to the columns either in a purified form, as root exudates from growing plants of Bt corn, or within the biomass of residues of Bt corn. The soil was amended to 0, 3, 6, 9, or 12% (vol vol-1) with the clays. Vertical movement of the protein generally decreased as the content of either clay was increased, and the amount of protein recovered in leachates increased as the concentration of purified protein added was increased. The largest amount of purified protein (ca. 75%) was leached from soil not amended with clay, whereas the lowest amount (ca. 16%) was recovered from columns containing soil amended to 12% with M or K. The Cry1Ab protein was also present in leachates from soil columns in which various hybrids of Bt corn were grown or to which biomass of Bt corn had been added, whereas it was absent in leachates from columns in which the respective isolines of non-Bt corn were grown or to which biomass of non-Bt corn had been added. The Cry1Ab protein exhibited stronger binding and higher persistence, as well as remaining nearer the soil surface, in soil that contained the higher clay concentrations (i.e. had a higher cation-exchange capacity and specific surface area), indicating that it could be transported to surface waters via runoff and erosion. In contrast, the protein was more readily leached through soil with lower clay concentrations, indicating that it could contaminate groundwater.

Original languageEnglish (US)
Pages (from-to)111-120
Number of pages10
JournalSoil Biology and Biochemistry
Volume34
Issue number1
DOIs
StatePublished - 2002

Fingerprint

soil movement
insecticidal proteins
vertical movement
Bacillus thuringiensis
Soil
Zea mays
Soils
protein
maize
clay
soil
Proteins
proteins
leachates
corn
Biomass
soil column
leachate
biomass
Plant Exudates

Keywords

  • Bacillus thuringiensis
  • Bt corn
  • Cry1Ab protein
  • Groundwater
  • Kaolinite
  • Leaching
  • Montmorillonite
  • Root exudates
  • Surfacewater

ASJC Scopus subject areas

  • Soil Science
  • Biochemistry
  • Ecology

Cite this

Vertical movement in soil of insecticidal Cry1Ab protein from Bacillus thuringiensis. / Saxena, Deepak; Flores, Saul; Stotzky, G.

In: Soil Biology and Biochemistry, Vol. 34, No. 1, 2002, p. 111-120.

Research output: Contribution to journalArticle

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abstract = "The effects of montmorillonite (M) or kaolinite (K) on the vertical movement of the insecticidal Cry1Ab protein of Bacillus thuringiensis subsp. kurstaki (Bt) were studied in repacked soil columns. The protein was added to the columns either in a purified form, as root exudates from growing plants of Bt corn, or within the biomass of residues of Bt corn. The soil was amended to 0, 3, 6, 9, or 12{\%} (vol vol-1) with the clays. Vertical movement of the protein generally decreased as the content of either clay was increased, and the amount of protein recovered in leachates increased as the concentration of purified protein added was increased. The largest amount of purified protein (ca. 75{\%}) was leached from soil not amended with clay, whereas the lowest amount (ca. 16{\%}) was recovered from columns containing soil amended to 12{\%} with M or K. The Cry1Ab protein was also present in leachates from soil columns in which various hybrids of Bt corn were grown or to which biomass of Bt corn had been added, whereas it was absent in leachates from columns in which the respective isolines of non-Bt corn were grown or to which biomass of non-Bt corn had been added. The Cry1Ab protein exhibited stronger binding and higher persistence, as well as remaining nearer the soil surface, in soil that contained the higher clay concentrations (i.e. had a higher cation-exchange capacity and specific surface area), indicating that it could be transported to surface waters via runoff and erosion. In contrast, the protein was more readily leached through soil with lower clay concentrations, indicating that it could contaminate groundwater.",
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