Use of tissue culture models to study neuronal regulatory trophic and toxic factors in the aged brain

Efrain C. Azmitia, Patricia M. Whitaker-Azmitia, Raymond Bartus

Research output: Contribution to journalArticle

Abstract

Dementia is believed to result from the loss of selective neurons within the brain, but approaches for systematic study of that degenerative process are hampered by the complexity of the neuronal milieu. Tissue culture models provide a means to reduce dramatically the variables inherent in the study of neuronal plasticity. Three levels of complexity can be described: cellular and molecular diveristy; primary and secondary interconnections; and finally, the dynamics influenced by age. The following review discusses the advantages and disadvantages of tissue culture models for the detailed study of neuronal trophic and toxic factors. Our selection of factors is broadened to include ions, intermediate metabolites, antioxidants, steroids, neuropeptides, gangliosides, metals, neurotransmitters, brain extracts, and protein molecules. Most of these factors have been shown to be altered in the aged brain, to have a significant effect on cultured neurons, or both. This multilevel analysis provides the reader with an overview of the events regulating neuronal survival, differentiation and death. An understanding of these basic questions is necessary to sequence the molecular events resulting in neuronal death.

Original languageEnglish (US)
Pages (from-to)743-758
Number of pages16
JournalNeurobiology of Aging
Volume9
Issue numberC
DOIs
StatePublished - 1988

Fingerprint

Poisons
Brain
Neurons
Multilevel Analysis
Neuronal Plasticity
Gangliosides
Neuropeptides
Neurotransmitter Agents
Dementia
Antioxidants
Metals
Steroids
Ions
Proteins

Keywords

  • Aging
  • Brain
  • Calcium Antioxidants
  • Growth factor
  • Neurotoxins
  • Tissue culture

ASJC Scopus subject areas

  • Aging
  • Developmental Biology
  • Geriatrics and Gerontology
  • Clinical Neurology
  • Neuroscience(all)
  • Biological Psychiatry
  • Developmental Neuroscience
  • Neurology
  • Psychology(all)

Cite this

Use of tissue culture models to study neuronal regulatory trophic and toxic factors in the aged brain. / Azmitia, Efrain C.; Whitaker-Azmitia, Patricia M.; Bartus, Raymond.

In: Neurobiology of Aging, Vol. 9, No. C, 1988, p. 743-758.

Research output: Contribution to journalArticle

Azmitia, Efrain C. ; Whitaker-Azmitia, Patricia M. ; Bartus, Raymond. / Use of tissue culture models to study neuronal regulatory trophic and toxic factors in the aged brain. In: Neurobiology of Aging. 1988 ; Vol. 9, No. C. pp. 743-758.
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