Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization

Marina Raguse, Marcel Fiebrandt, Benjamin Denis, Katharina Stapelmann, Patrick Eichenberger, Adam Driks, Peter Eaton, Peter Awakowicz, Ralf Moeller

Research output: Contribution to journalArticle

Abstract

Low-pressure plasmas have been evaluated for their potential in biomedical and defense purposes. The sterilizing effect of plasma can be attributed to several active agents, including (V)UV radiation, charged particles, radical species, neutral and excited atoms and molecules, and the electric field. Spores of Bacillus subtilis were used as a bioindicator and a genetic model system to study the sporicidal effects of low-pressure plasma decontamination. Wild-type spores, spores lacking the major protective coat layers (inner, outer, and crust), pigmentation-deficient spores or spore impaired in encasement (a late step in coat assembly) were systematically tested for their resistance to low-pressure argon, hydrogen, and oxygen plasmas with and without admixtures. We demonstrate that low-pressure plasma discharges of argon and oxygen discharges cause significant physical damage to spore surface structures as visualized by atomic force microscopy. Spore resistance to low-pressure plasma was primarily dependent on the presence of the inner, and outer spore coat layers as well as spore encasement, with minor or less importance of the crust and spore pigmentation, whereas spore inactivation itself was strongly influenced by the gas composition and operational settings.

Original languageEnglish (US)
Article number285401
JournalJournal of Physics D: Applied Physics
Volume49
Issue number28
DOIs
StatePublished - Jun 15 2016

Fingerprint

spores
Bacillus
Bacilli
low pressure
Plasmas
Argon
Oxygen
Decontamination
Biomarkers
Charged particles
Surface structure
Ultraviolet radiation
crusts
Hydrogen
Atomic force microscopy
Gases
Electric fields
decontamination
Atoms
gas composition

Keywords

  • Bacillus subtilis
  • bioindicator
  • decontamination
  • plasma sterilization
  • spore coat
  • spore resistance
  • spores

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Acoustics and Ultrasonics
  • Surfaces, Coatings and Films

Cite this

Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization. / Raguse, Marina; Fiebrandt, Marcel; Denis, Benjamin; Stapelmann, Katharina; Eichenberger, Patrick; Driks, Adam; Eaton, Peter; Awakowicz, Peter; Moeller, Ralf.

In: Journal of Physics D: Applied Physics, Vol. 49, No. 28, 285401, 15.06.2016.

Research output: Contribution to journalArticle

Raguse, Marina ; Fiebrandt, Marcel ; Denis, Benjamin ; Stapelmann, Katharina ; Eichenberger, Patrick ; Driks, Adam ; Eaton, Peter ; Awakowicz, Peter ; Moeller, Ralf. / Understanding of the importance of the spore coat structure and pigmentation in the Bacillus subtilis spore resistance to low-pressure plasma sterilization. In: Journal of Physics D: Applied Physics. 2016 ; Vol. 49, No. 28.
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