Uncovering buffered pleiotropy: A genome-scale screen for mel-28 genetic interactors in caenorhabditis elegans

Anita G. Fernandez, Emily K. Mis, Allison Lai, Michael Mauro, Angela Quental, Carly Bock, Fabio Piano

Research output: Contribution to journalArticle

Abstract

mel-28 (maternal-effect-lethal-28) encodes a conserved protein required for nuclear envelope function and chromosome segregation in Caenorhabditis elegans. Because mel-28 is a strict maternal-effect lethal gene, its function is required in the early embryo but appears to be dispensable for larval development. We wanted to test the idea that mel-28 has postembryonic roles that are buffered by the contributions of other genes. To find genes that act coordinately with mel-28, we did an RNA interference2based genetic interaction screen using mel-28 and wild-type larvae. We screened 18,364 clones and identified 65 genes that cause sterility in mel-28 but not wild-type worms. Some of these genes encode components of the nuclear pore. In addition we identified genes involved in dynein and dynactin function, vesicle transport, and cell-matrix attachments. By screening mel-28 larvae we have bypassed the requirement for mel-28 in the embryo, uncovering pleiotropic functions for mel-28 later in development that are normally provided by other genes. This work contributes toward revealing the gene networks that underlie cellular processes and reveals roles for a maternal-effect lethal gene later in development.

Original languageEnglish (US)
Pages (from-to)185-196
Number of pages12
JournalG3: Genes, Genomes, Genetics
Volume4
Issue number1
DOIs
StatePublished - 2014

Fingerprint

Caenorhabditis elegans
Genome
Lethal Genes
Genes
Larva
Maternal Inheritance
Embryonic Structures
Gene Components
Nuclear Pore
Dyneins
Transport Vesicles
Chromosome Segregation
Gene Regulatory Networks
Nuclear Envelope
Infertility
Clone Cells
RNA

Keywords

  • C. elegans
  • Germline
  • Gonad
  • mel-28
  • Synthetic sterility

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

Uncovering buffered pleiotropy : A genome-scale screen for mel-28 genetic interactors in caenorhabditis elegans. / Fernandez, Anita G.; Mis, Emily K.; Lai, Allison; Mauro, Michael; Quental, Angela; Bock, Carly; Piano, Fabio.

In: G3: Genes, Genomes, Genetics, Vol. 4, No. 1, 2014, p. 185-196.

Research output: Contribution to journalArticle

Fernandez, Anita G. ; Mis, Emily K. ; Lai, Allison ; Mauro, Michael ; Quental, Angela ; Bock, Carly ; Piano, Fabio. / Uncovering buffered pleiotropy : A genome-scale screen for mel-28 genetic interactors in caenorhabditis elegans. In: G3: Genes, Genomes, Genetics. 2014 ; Vol. 4, No. 1. pp. 185-196.
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