Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes

Olaf F A Larsen, Ivo H M Van Stokkum, Frank L. De Weerd, Mikas Vengris, Charuvila T. Aravindakumar, Rienk Van Grondelle, Nicholas Geacintov, Herbert Van Amerongen

Research output: Contribution to journalArticle

Abstract

Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the steady-state fluorescence was observed in all samples. The transient-absorption spectra were remarkably similar to those of the isolated 2-aminopurine (Larsen et al.; O. F. A. Larsen, I. H. M. van Stokkum, M.-L. Groot, J. T. M. Kennis, R. van Grondelle and H. van Amerongen, Chem. Phys. Lett., 2003, 371, 157-163), exhibiting both a fluorescent and a non-fluorescent excited state. There was no evidence for significant amounts of charge-separated states in the transient-absorption spectra. The probability that an excitation of 2AP leads to stable charge transfer products was estimated to be very low (∼0.1%). In the systems we studied, the observed fluorescence quenching can largely be explained by a shift of the equilibrium between the two excited states in 2AP, in which the non-fluorescent state is favored.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalPhysical Chemistry Chemical Physics
Volume6
Issue number1
DOIs
StatePublished - Jan 7 2004

Fingerprint

2-Aminopurine
deoxyribonucleic acid
Fluorescence
adenines
Adenine
Excited states
fluorescence
Absorption spectra
Quenching
DNA
quenching
excitation
absorption spectra
Hypoxanthine
Thymine
thymine
guanines
Oligodeoxyribonucleotides
Cytosine
Guanine

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Atomic and Molecular Physics, and Optics

Cite this

Larsen, O. F. A., Van Stokkum, I. H. M., De Weerd, F. L., Vengris, M., Aravindakumar, C. T., Van Grondelle, R., ... Van Amerongen, H. (2004). Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes. Physical Chemistry Chemical Physics, 6(1), 154-160. https://doi.org/10.1039/b308992d

Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes. / Larsen, Olaf F A; Van Stokkum, Ivo H M; De Weerd, Frank L.; Vengris, Mikas; Aravindakumar, Charuvila T.; Van Grondelle, Rienk; Geacintov, Nicholas; Van Amerongen, Herbert.

In: Physical Chemistry Chemical Physics, Vol. 6, No. 1, 07.01.2004, p. 154-160.

Research output: Contribution to journalArticle

Larsen, Olaf F A ; Van Stokkum, Ivo H M ; De Weerd, Frank L. ; Vengris, Mikas ; Aravindakumar, Charuvila T. ; Van Grondelle, Rienk ; Geacintov, Nicholas ; Van Amerongen, Herbert. / Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes. In: Physical Chemistry Chemical Physics. 2004 ; Vol. 6, No. 1. pp. 154-160.
@article{a1b08dc31c6d4f9e85165e16d4536208,
title = "Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes",
abstract = "Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the steady-state fluorescence was observed in all samples. The transient-absorption spectra were remarkably similar to those of the isolated 2-aminopurine (Larsen et al.; O. F. A. Larsen, I. H. M. van Stokkum, M.-L. Groot, J. T. M. Kennis, R. van Grondelle and H. van Amerongen, Chem. Phys. Lett., 2003, 371, 157-163), exhibiting both a fluorescent and a non-fluorescent excited state. There was no evidence for significant amounts of charge-separated states in the transient-absorption spectra. The probability that an excitation of 2AP leads to stable charge transfer products was estimated to be very low (∼0.1{\%}). In the systems we studied, the observed fluorescence quenching can largely be explained by a shift of the equilibrium between the two excited states in 2AP, in which the non-fluorescent state is favored.",
author = "Larsen, {Olaf F A} and {Van Stokkum}, {Ivo H M} and {De Weerd}, {Frank L.} and Mikas Vengris and Aravindakumar, {Charuvila T.} and {Van Grondelle}, Rienk and Nicholas Geacintov and {Van Amerongen}, Herbert",
year = "2004",
month = "1",
day = "7",
doi = "10.1039/b308992d",
language = "English (US)",
volume = "6",
pages = "154--160",
journal = "Physical Chemistry Chemical Physics",
issn = "1463-9076",
publisher = "Royal Society of Chemistry",
number = "1",

}

TY - JOUR

T1 - Ultrafast transient-absorption and steady-state fluorescence measurements on 2-aminopurine substituted dinucleotides and 2-aminopurine substituted DNA duplexes

AU - Larsen, Olaf F A

AU - Van Stokkum, Ivo H M

AU - De Weerd, Frank L.

AU - Vengris, Mikas

AU - Aravindakumar, Charuvila T.

AU - Van Grondelle, Rienk

AU - Geacintov, Nicholas

AU - Van Amerongen, Herbert

PY - 2004/1/7

Y1 - 2004/1/7

N2 - Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the steady-state fluorescence was observed in all samples. The transient-absorption spectra were remarkably similar to those of the isolated 2-aminopurine (Larsen et al.; O. F. A. Larsen, I. H. M. van Stokkum, M.-L. Groot, J. T. M. Kennis, R. van Grondelle and H. van Amerongen, Chem. Phys. Lett., 2003, 371, 157-163), exhibiting both a fluorescent and a non-fluorescent excited state. There was no evidence for significant amounts of charge-separated states in the transient-absorption spectra. The probability that an excitation of 2AP leads to stable charge transfer products was estimated to be very low (∼0.1%). In the systems we studied, the observed fluorescence quenching can largely be explained by a shift of the equilibrium between the two excited states in 2AP, in which the non-fluorescent state is favored.

AB - Ultrafast transient-absorption and steady-state fluorescence measurements have been performed on dinucleotides comprising the fluorescent adenine analogue 2-aminopurine and guanine, adenine, cytosine, thymine or hypoxanthine, respectively. Two oligodeoxyribonucleotide duplexes that were site-selectively substituted with a single 2-aminopurine moiety were also studied. A strong quenching of the steady-state fluorescence was observed in all samples. The transient-absorption spectra were remarkably similar to those of the isolated 2-aminopurine (Larsen et al.; O. F. A. Larsen, I. H. M. van Stokkum, M.-L. Groot, J. T. M. Kennis, R. van Grondelle and H. van Amerongen, Chem. Phys. Lett., 2003, 371, 157-163), exhibiting both a fluorescent and a non-fluorescent excited state. There was no evidence for significant amounts of charge-separated states in the transient-absorption spectra. The probability that an excitation of 2AP leads to stable charge transfer products was estimated to be very low (∼0.1%). In the systems we studied, the observed fluorescence quenching can largely be explained by a shift of the equilibrium between the two excited states in 2AP, in which the non-fluorescent state is favored.

UR - http://www.scopus.com/inward/record.url?scp=1642533609&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642533609&partnerID=8YFLogxK

U2 - 10.1039/b308992d

DO - 10.1039/b308992d

M3 - Article

VL - 6

SP - 154

EP - 160

JO - Physical Chemistry Chemical Physics

JF - Physical Chemistry Chemical Physics

SN - 1463-9076

IS - 1

ER -