Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts

Yorimasa Ogata, Sumi Nakao, Emi Shimizu, Yuko Matsuda-Honjyo, Muneyoshi Yamazaki, Shunsuke Furuyama, Hiroshi Sugiya

Research output: Contribution to journalArticle

Abstract

Bradykinin (1 μM) and histamine (100 μM) evoked an initial transient increase and a subsequent sustained increase in intracellular Ca2+concentration ([Ca2+]i) in fura-2-loaded human gingival fibroblasts, which may be attributed to Ca2+release from intracellular stores and Ca2+entry from extracellular sites, respectively. In fibroblasts pretreated with tyrosine kinase inhibitors such as herbimycin A (1 μM) and tyrphostin 47 (20 μM),the sustained level of [Ca2+]iinduced by bradykinin and histamine increased, but not the initial peak level. In the absence of external Ca2+, bradykinin and histamine induced only the transient increase in [Ca2+]i, but a subsequent addition of Ca2+to the medium resulted in a sustained increase in [Ca2+]icaused by Ca2+entry. Thapsigargin, an inhibitor of Ca2+-ATPase in inositol 1,4,5-trisphosphate-sensitive Ca2+stores, mimicked the effect of bradykinin and histamine. In the fibroblasts pretreated with tyrosine kinase inhibitors, the bradykinin-, histamine- and thapsigargin-induced Ca2+entry was clearly enhanced, but not the transient [Ca2+]iincrease. Tyrosine phosphatase inhibitor benzylphosphonic acid (200 μM) had no effect on Ca2+entry or transient [Ca2+]iincrease. These results suggest that tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts.

Original languageEnglish (US)
Pages (from-to)689-693
Number of pages5
JournalCell Biology International
Volume27
Issue number8
DOIs
StatePublished - Aug 1 2003

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Bradykinin
Histamine
Tyrosine
Fibroblasts
Phosphorylation
Thapsigargin
Protein-Tyrosine Kinases
Inositol 1,4,5-Trisphosphate
Calcium-Transporting ATPases
Fura-2
Phosphoric Monoester Hydrolases

Keywords

  • Caentry
  • Human gingival fibroblasts
  • Tyrosine kinase inhibitor
  • Tyrosine phosphorylation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ogata, Y., Nakao, S., Shimizu, E., Matsuda-Honjyo, Y., Yamazaki, M., Furuyama, S., & Sugiya, H. (2003). Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts. Cell Biology International, 27(8), 689-693. https://doi.org/10.1016/S1065-6995(03)00125-2

Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts. / Ogata, Yorimasa; Nakao, Sumi; Shimizu, Emi; Matsuda-Honjyo, Yuko; Yamazaki, Muneyoshi; Furuyama, Shunsuke; Sugiya, Hiroshi.

In: Cell Biology International, Vol. 27, No. 8, 01.08.2003, p. 689-693.

Research output: Contribution to journalArticle

Ogata, Y, Nakao, S, Shimizu, E, Matsuda-Honjyo, Y, Yamazaki, M, Furuyama, S & Sugiya, H 2003, 'Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts', Cell Biology International, vol. 27, no. 8, pp. 689-693. https://doi.org/10.1016/S1065-6995(03)00125-2
Ogata Y, Nakao S, Shimizu E, Matsuda-Honjyo Y, Yamazaki M, Furuyama S et al. Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts. Cell Biology International. 2003 Aug 1;27(8):689-693. https://doi.org/10.1016/S1065-6995(03)00125-2
Ogata, Yorimasa ; Nakao, Sumi ; Shimizu, Emi ; Matsuda-Honjyo, Yuko ; Yamazaki, Muneyoshi ; Furuyama, Shunsuke ; Sugiya, Hiroshi. / Tyrosine phosphorylation is involved in Ca2+entry in human gingival fibroblasts. In: Cell Biology International. 2003 ; Vol. 27, No. 8. pp. 689-693.
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