Two phases of astral microtubule activity during cytokinesis in C. elegans embryos

Fumio Motegi, Nathalie V. Velarde, Fabio Piano, Asako Sugimoto

Research output: Contribution to journalArticle

Abstract

Microtubules of the mitotic spindle are believed to provide positional cues for the assembly of the actin-based contractile ring and the formation of the subsequent cleavage furrow during cytokinesis. In Caenorhabditis elegans, astral microtubules have been thought to inhibit cortical contraction outside the cleavage furrow. Here, we demonstrate by live imaging and RNA interference (RNAi) that astral microtubules play two distinct roles in initiating cleavage furrow formation. In early anaphase, microtubules are required for contractile ring assembly; in late anaphase, microtubules show different cortical behavior and seem to suppress cortical contraction at the poles, as suggested in previous studies. These two distinct phases of microtubule behavior depend on distinct regulatory pathways, one involving the γ-tubulin complex and the other requiring aurora-A kinase. We propose that temporal and spatial regulation of two distinct phases of astral microtubule behavior is crucial in specifying the position and timing of furrowing.

Original languageEnglish (US)
Pages (from-to)509-520
Number of pages12
JournalDevelopmental Cell
Volume10
Issue number4
DOIs
StatePublished - Apr 2006

Fingerprint

Cytokinesis
Microtubules
Embryonic Structures
Aurora Kinase A
Tubulin
Actins
Poles
RNA
Anaphase
Imaging techniques
Spindle Apparatus
Caenorhabditis elegans
RNA Interference
Cues

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Two phases of astral microtubule activity during cytokinesis in C. elegans embryos. / Motegi, Fumio; Velarde, Nathalie V.; Piano, Fabio; Sugimoto, Asako.

In: Developmental Cell, Vol. 10, No. 4, 04.2006, p. 509-520.

Research output: Contribution to journalArticle

Motegi, Fumio ; Velarde, Nathalie V. ; Piano, Fabio ; Sugimoto, Asako. / Two phases of astral microtubule activity during cytokinesis in C. elegans embryos. In: Developmental Cell. 2006 ; Vol. 10, No. 4. pp. 509-520.
@article{dc507cd1e94045618fa65bdb44711bb9,
title = "Two phases of astral microtubule activity during cytokinesis in C. elegans embryos",
abstract = "Microtubules of the mitotic spindle are believed to provide positional cues for the assembly of the actin-based contractile ring and the formation of the subsequent cleavage furrow during cytokinesis. In Caenorhabditis elegans, astral microtubules have been thought to inhibit cortical contraction outside the cleavage furrow. Here, we demonstrate by live imaging and RNA interference (RNAi) that astral microtubules play two distinct roles in initiating cleavage furrow formation. In early anaphase, microtubules are required for contractile ring assembly; in late anaphase, microtubules show different cortical behavior and seem to suppress cortical contraction at the poles, as suggested in previous studies. These two distinct phases of microtubule behavior depend on distinct regulatory pathways, one involving the γ-tubulin complex and the other requiring aurora-A kinase. We propose that temporal and spatial regulation of two distinct phases of astral microtubule behavior is crucial in specifying the position and timing of furrowing.",
author = "Fumio Motegi and Velarde, {Nathalie V.} and Fabio Piano and Asako Sugimoto",
year = "2006",
month = "4",
doi = "10.1016/j.devcel.2006.03.001",
language = "English (US)",
volume = "10",
pages = "509--520",
journal = "Developmental Cell",
issn = "1534-5807",
publisher = "Cell Press",
number = "4",

}

TY - JOUR

T1 - Two phases of astral microtubule activity during cytokinesis in C. elegans embryos

AU - Motegi, Fumio

AU - Velarde, Nathalie V.

AU - Piano, Fabio

AU - Sugimoto, Asako

PY - 2006/4

Y1 - 2006/4

N2 - Microtubules of the mitotic spindle are believed to provide positional cues for the assembly of the actin-based contractile ring and the formation of the subsequent cleavage furrow during cytokinesis. In Caenorhabditis elegans, astral microtubules have been thought to inhibit cortical contraction outside the cleavage furrow. Here, we demonstrate by live imaging and RNA interference (RNAi) that astral microtubules play two distinct roles in initiating cleavage furrow formation. In early anaphase, microtubules are required for contractile ring assembly; in late anaphase, microtubules show different cortical behavior and seem to suppress cortical contraction at the poles, as suggested in previous studies. These two distinct phases of microtubule behavior depend on distinct regulatory pathways, one involving the γ-tubulin complex and the other requiring aurora-A kinase. We propose that temporal and spatial regulation of two distinct phases of astral microtubule behavior is crucial in specifying the position and timing of furrowing.

AB - Microtubules of the mitotic spindle are believed to provide positional cues for the assembly of the actin-based contractile ring and the formation of the subsequent cleavage furrow during cytokinesis. In Caenorhabditis elegans, astral microtubules have been thought to inhibit cortical contraction outside the cleavage furrow. Here, we demonstrate by live imaging and RNA interference (RNAi) that astral microtubules play two distinct roles in initiating cleavage furrow formation. In early anaphase, microtubules are required for contractile ring assembly; in late anaphase, microtubules show different cortical behavior and seem to suppress cortical contraction at the poles, as suggested in previous studies. These two distinct phases of microtubule behavior depend on distinct regulatory pathways, one involving the γ-tubulin complex and the other requiring aurora-A kinase. We propose that temporal and spatial regulation of two distinct phases of astral microtubule behavior is crucial in specifying the position and timing of furrowing.

UR - http://www.scopus.com/inward/record.url?scp=33645521057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645521057&partnerID=8YFLogxK

U2 - 10.1016/j.devcel.2006.03.001

DO - 10.1016/j.devcel.2006.03.001

M3 - Article

C2 - 16580995

AN - SCOPUS:33645521057

VL - 10

SP - 509

EP - 520

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 4

ER -