Two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome

Robert C. Murphy, Joyce J. Diwan, Michael King, Kathleen W. Kinnally

Research output: Contribution to journalArticle

Abstract

Patch-clamp techniques were used to characterize the channel activity of mitochondrial inner membranes of two human osteosarcoma cell lines: a mitochondrial genome-deficient (ρ 0) line and its corresponding parental (ρ +) line. Previously, two high conductance channels, mitochondrial Centum picoSiemen (mCS) and multiple conductance channels (MCC), were detected in murine mitochondria. While MCC was assigned to the protein import in yeast mitochondria, the role of mCS is unknown. This study demonstrates that mCs and MCC activities from mouse mitochondria are indistinguishable from those of human mitochondria. The channel activities and their functional expression levels are not altered in cells lacking mtDNA. Hence, ρ 0 cells may provide a model system for elucidating the role of mitochondrial channels in disease processes and apoptosis.

Original languageEnglish (US)
Pages (from-to)259-262
Number of pages4
JournalFEBS Letters
Volume425
Issue number2
DOIs
StatePublished - Mar 27 1998

Fingerprint

Mitochondria
Mitochondrial Genome
Mitochondrial Membranes
Human Genome
Genes
Membranes
Clamping devices
Patch-Clamp Techniques
Osteosarcoma
Mitochondrial DNA
Yeast
Yeasts
Cells
Apoptosis
Cell Line
Proteins

Keywords

  • ρ
  • Channel
  • Mitochondrial DNA
  • Mitochondrion
  • Multiple conductance channel activity
  • Patch-clamp

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome. / Murphy, Robert C.; Diwan, Joyce J.; King, Michael; Kinnally, Kathleen W.

In: FEBS Letters, Vol. 425, No. 2, 27.03.1998, p. 259-262.

Research output: Contribution to journalArticle

Murphy, Robert C. ; Diwan, Joyce J. ; King, Michael ; Kinnally, Kathleen W. / Two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome. In: FEBS Letters. 1998 ; Vol. 425, No. 2. pp. 259-262.
@article{ed9f8fdcd3c246f3a9d50a161e7caf56,
title = "Two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome",
abstract = "Patch-clamp techniques were used to characterize the channel activity of mitochondrial inner membranes of two human osteosarcoma cell lines: a mitochondrial genome-deficient (ρ 0) line and its corresponding parental (ρ +) line. Previously, two high conductance channels, mitochondrial Centum picoSiemen (mCS) and multiple conductance channels (MCC), were detected in murine mitochondria. While MCC was assigned to the protein import in yeast mitochondria, the role of mCS is unknown. This study demonstrates that mCs and MCC activities from mouse mitochondria are indistinguishable from those of human mitochondria. The channel activities and their functional expression levels are not altered in cells lacking mtDNA. Hence, ρ 0 cells may provide a model system for elucidating the role of mitochondrial channels in disease processes and apoptosis.",
keywords = "ρ, Channel, Mitochondrial DNA, Mitochondrion, Multiple conductance channel activity, Patch-clamp",
author = "Murphy, {Robert C.} and Diwan, {Joyce J.} and Michael King and Kinnally, {Kathleen W.}",
year = "1998",
month = "3",
day = "27",
doi = "10.1016/S0014-5793(98)00245-2",
language = "English (US)",
volume = "425",
pages = "259--262",
journal = "FEBS Letters",
issn = "0014-5793",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Two high conductance channels of the mitochondrial inner membrane are independent of the human mitochondrial genome

AU - Murphy, Robert C.

AU - Diwan, Joyce J.

AU - King, Michael

AU - Kinnally, Kathleen W.

PY - 1998/3/27

Y1 - 1998/3/27

N2 - Patch-clamp techniques were used to characterize the channel activity of mitochondrial inner membranes of two human osteosarcoma cell lines: a mitochondrial genome-deficient (ρ 0) line and its corresponding parental (ρ +) line. Previously, two high conductance channels, mitochondrial Centum picoSiemen (mCS) and multiple conductance channels (MCC), were detected in murine mitochondria. While MCC was assigned to the protein import in yeast mitochondria, the role of mCS is unknown. This study demonstrates that mCs and MCC activities from mouse mitochondria are indistinguishable from those of human mitochondria. The channel activities and their functional expression levels are not altered in cells lacking mtDNA. Hence, ρ 0 cells may provide a model system for elucidating the role of mitochondrial channels in disease processes and apoptosis.

AB - Patch-clamp techniques were used to characterize the channel activity of mitochondrial inner membranes of two human osteosarcoma cell lines: a mitochondrial genome-deficient (ρ 0) line and its corresponding parental (ρ +) line. Previously, two high conductance channels, mitochondrial Centum picoSiemen (mCS) and multiple conductance channels (MCC), were detected in murine mitochondria. While MCC was assigned to the protein import in yeast mitochondria, the role of mCS is unknown. This study demonstrates that mCs and MCC activities from mouse mitochondria are indistinguishable from those of human mitochondria. The channel activities and their functional expression levels are not altered in cells lacking mtDNA. Hence, ρ 0 cells may provide a model system for elucidating the role of mitochondrial channels in disease processes and apoptosis.

KW - ρ

KW - Channel

KW - Mitochondrial DNA

KW - Mitochondrion

KW - Multiple conductance channel activity

KW - Patch-clamp

UR - http://www.scopus.com/inward/record.url?scp=0032571420&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032571420&partnerID=8YFLogxK

U2 - 10.1016/S0014-5793(98)00245-2

DO - 10.1016/S0014-5793(98)00245-2

M3 - Article

VL - 425

SP - 259

EP - 262

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -