Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers

Jarrod J. Buffy, Nathaniel Traaseth, Alessandro Mascioni, Peter L. Gor'kov, Eduard Y. Chekmenev, William W. Brey, Gianluigi Veglia

Research output: Contribution to journalArticle

Abstract

Sarcolipin (SLN), a 31 amino acid integral membrane protein, regulates SERCA1a and SERCA2a, two isoforms of the sarco(endo)plasmic Ca-ATPase, by lowering their apparent Ca2+ affinity and thereby enabling muscle relaxation. SLN is expressed in both fast-twitch and slow-twitch muscle fibers with significant expression levels also found in the cardiac muscle. SLN shares ∼30% identity with the transmembrane domain of phospholamban (PLN), and recent solution NMR studies carried out in detergent micelles indicate that the two polypeptides bind to SERCA in a similar manner. Previous ID solid-state NMR experiments on selectively 15N-labeled sites showed that SLN crosses the lipid bilayer with an orientation nearly parallel to the bilayer normal. With a view toward the characterization of SLN structure and its interactions with both lipids and SERCA, herein we report our initial structural and topological assignments of SLN in mechanically oriented DOPC/DOPE lipid bilayers as mapped by 2D 15N PISEMA experiments. The PISEMA spectra obtained on uniformly 15N-labeled protein as well as 15N-Leu, 15N-Ile and 15N-Val map the secondary structure of SLN and, simultaneously, reveal that SLN exists in two distinct topologies. Both the major and the minor populations assume an orientation with the helix axis tilted by ∼23° with respect to the lipid bilayer normal, but vary in the rotation angle about the helix axis by ∼5°. The existence of the multiple populations in model membranes may be a significant requirement for SLN interaction with SERCA.

Original languageEnglish (US)
Pages (from-to)10939-10946
Number of pages8
JournalBiochemistry
Volume45
Issue number36
DOIs
StatePublished - Sep 12 2006

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Lipid bilayers
Lipid Bilayers
Nuclear magnetic resonance
Topology
Muscle
Slow-Twitch Muscle Fibers
sarcolipin
Muscle Relaxation
Micelles
Detergents
Population
Adenosine Triphosphatases
Myocardium
Protein Isoforms
Membrane Proteins
Experiments
Membranes
Lipids
Amino Acids
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Buffy, J. J., Traaseth, N., Mascioni, A., Gor'kov, P. L., Chekmenev, E. Y., Brey, W. W., & Veglia, G. (2006). Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers. Biochemistry, 45(36), 10939-10946. https://doi.org/10.1021/bi060728d

Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers. / Buffy, Jarrod J.; Traaseth, Nathaniel; Mascioni, Alessandro; Gor'kov, Peter L.; Chekmenev, Eduard Y.; Brey, William W.; Veglia, Gianluigi.

In: Biochemistry, Vol. 45, No. 36, 12.09.2006, p. 10939-10946.

Research output: Contribution to journalArticle

Buffy, JJ, Traaseth, N, Mascioni, A, Gor'kov, PL, Chekmenev, EY, Brey, WW & Veglia, G 2006, 'Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers', Biochemistry, vol. 45, no. 36, pp. 10939-10946. https://doi.org/10.1021/bi060728d
Buffy, Jarrod J. ; Traaseth, Nathaniel ; Mascioni, Alessandro ; Gor'kov, Peter L. ; Chekmenev, Eduard Y. ; Brey, William W. ; Veglia, Gianluigi. / Two-dimensional solid-state NMR reveals two topologies of sarcolipin in oriented lipid bilayers. In: Biochemistry. 2006 ; Vol. 45, No. 36. pp. 10939-10946.
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