Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

Alexis Gambis, Pierre Dourlen, Hermann Steller, Bertrand Mollereau

    Research output: Contribution to journalArticle

    Abstract

    We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

    Original languageEnglish (US)
    Pages (from-to)128-134
    Number of pages7
    JournalDevelopmental Biology
    Volume351
    Issue number1
    DOIs
    StatePublished - Mar 1 2011

    Fingerprint

    Drosophila
    Cell Polarity
    Color
    Apoptosis
    Neurons
    Nerve Degeneration
    Caspase Inhibitors
    Lycopersicon esculentum
    Caspases
    Diptera
    Cell Differentiation
    Mutation
    Genes

    Keywords

    • Apoptosis
    • Development
    • Neuron
    • Polarity
    • Retina

    ASJC Scopus subject areas

    • Molecular Biology
    • Developmental Biology
    • Cell Biology

    Cite this

    Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. / Gambis, Alexis; Dourlen, Pierre; Steller, Hermann; Mollereau, Bertrand.

    In: Developmental Biology, Vol. 351, No. 1, 01.03.2011, p. 128-134.

    Research output: Contribution to journalArticle

    Gambis, Alexis ; Dourlen, Pierre ; Steller, Hermann ; Mollereau, Bertrand. / Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. In: Developmental Biology. 2011 ; Vol. 351, No. 1. pp. 128-134.
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