Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila

Alexis Gambis, Pierre Dourlen, Hermann Steller, Bertrand Mollereau

Research output: Contribution to journalArticle

Abstract

We report a new two-color fluorescent imaging system to visualize the mosaic adult photoreceptor neurons (PRs) in real-time. Using this method, we examined a collection of 434 mutants and identified genes required for PR survival, planar cell polarity (PCP), patterning and differentiation. We could track the progression of PR degeneration in living flies. By introducing the expression of p35, a caspase inhibitor, we found mutations that specifically activate caspase-dependent death. Moreover, we showed that grh is required in R3 for correct PCP establishment. The "Tomato/GFP-FLP/FRT" method allows high-throughput, rapid and precise identification of survival and developmental pathways in living adult PRs at single-cell resolution.

Original languageEnglish (US)
Pages (from-to)128-134
Number of pages7
JournalDevelopmental Biology
Volume351
Issue number1
DOIs
StatePublished - Mar 1 2011

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Drosophila
Cell Polarity
Color
Apoptosis
Neurons
Nerve Degeneration
Caspase Inhibitors
Lycopersicon esculentum
Caspases
Diptera
Cell Differentiation
Mutation
Genes

Keywords

  • Apoptosis
  • Development
  • Neuron
  • Polarity
  • Retina

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. / Gambis, Alexis; Dourlen, Pierre; Steller, Hermann; Mollereau, Bertrand.

In: Developmental Biology, Vol. 351, No. 1, 01.03.2011, p. 128-134.

Research output: Contribution to journalArticle

Gambis, Alexis ; Dourlen, Pierre ; Steller, Hermann ; Mollereau, Bertrand. / Two-color in vivo imaging of photoreceptor apoptosis and development in Drosophila. In: Developmental Biology. 2011 ; Vol. 351, No. 1. pp. 128-134.
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