Two-aperture microfluidic probes as flow dipole

Theory and applications

Mohammadali Safavieh, Mohammad Qasaimeh, Ali Vakil, David Juncker, Thomas Gervais

    Research output: Contribution to journalArticle

    Abstract

    A microfluidic probe (MFP) is a mobile channel-less microfluidic system under which a fluid is injected from an aperture into an open space, hydrodynamically confined by a surrounding fluid, and entirely re-aspirated into a second aperture. Various MFPs have been developed, and have been used for applications ranging from surface patterning of photoresists to local perfusion of organotypic tissue slices. However, the hydrodynamic and mass transfer properties of the flow under the MFP have not been analyzed, and the flow parameters are adjusted empirically. Here, we present an analytical model describing the key transport properties in MFP operation, including the dimensions of the hydrodynamic flow confinement (HFC) area, diffusion broadening, and shear stress as a function of: (i) probe geometry (ii) aspiration-to-injection flow rate ratio (iii) gap between MFP and substrate and (iv) reagent diffusivity. Analytical results and scaling laws were validated against numerical simulations and experimental results from published data. These results will be useful to guide future MFP design and operation, notably to control the MFP "brush stroke" while preserving shear-sensitive cells and tissues.

    Original languageEnglish (US)
    Article number11943
    JournalScientific Reports
    Volume5
    DOIs
    StatePublished - Jul 14 2015

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    Microfluidics
    Hydrodynamics
    Confined Spaces
    Perfusion
    Stroke
    Injections

    ASJC Scopus subject areas

    • General

    Cite this

    Safavieh, M., Qasaimeh, M., Vakil, A., Juncker, D., & Gervais, T. (2015). Two-aperture microfluidic probes as flow dipole: Theory and applications. Scientific Reports, 5, [11943]. https://doi.org/10.1038/srep11943

    Two-aperture microfluidic probes as flow dipole : Theory and applications. / Safavieh, Mohammadali; Qasaimeh, Mohammad; Vakil, Ali; Juncker, David; Gervais, Thomas.

    In: Scientific Reports, Vol. 5, 11943, 14.07.2015.

    Research output: Contribution to journalArticle

    Safavieh, M, Qasaimeh, M, Vakil, A, Juncker, D & Gervais, T 2015, 'Two-aperture microfluidic probes as flow dipole: Theory and applications', Scientific Reports, vol. 5, 11943. https://doi.org/10.1038/srep11943
    Safavieh, Mohammadali ; Qasaimeh, Mohammad ; Vakil, Ali ; Juncker, David ; Gervais, Thomas. / Two-aperture microfluidic probes as flow dipole : Theory and applications. In: Scientific Reports. 2015 ; Vol. 5.
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