Twinstar, the Drosophila homolog of cofilin/ADF, is required for planar cell polarity patterning

Adrienne Blair, Andrew Tomlinson, Hung Pham, Kristin C. Gunsalus, Michael L. Goldberg, Frank A. Laski

Research output: Contribution to journalArticle

Abstract

Planar cell polarity (PCP) is a level of tissue organization in which cells adopt a uniform orientation within the plane of an epithelium. The process of tissue polarization is likely to be initiated by an extracellular gradient. Thus, determining how cells decode and convert this graded information into subcellular asymmetries is key to determining how cells direct the reorganization of the cytoskeleton to produce uniformly oriented structures. Twinstar (Tsr), the Drosophila homolog of Cofilin/ADF (actin depolymerization factor), is a component of the cytoskeleton that regulates actin dynamics. We show here that various alleles of tsr produce PCP defects in the wing, eye and several other epithelia. In wings mutant for tsr, Frizzled (Fz) and Flamingo (Fmi) proteins do not properly localize to the proximodistal boundaries of cells. The correct asymmetric localization of these proteins instructs the actin cytoskeleton to produce one actin-rich wing hair at the distal-most vertex of each cell. These results argue that actin remodeling is not only required in the manufacture of wing hairs, but also in the PCP read-out that directs where a wing hair will be secreted.

Original languageEnglish (US)
Pages (from-to)1789-1797
Number of pages9
JournalDevelopment
Volume133
Issue number9
DOIs
StatePublished - May 2006

Fingerprint

Actin Depolymerizing Factors
Cell Polarity
Drosophila
Actins
Actin Cytoskeleton
Epithelium
Frizzled Receptors
Cytoskeleton
Alleles
Proteins

Keywords

  • ADF/cofilin
  • Drosophila
  • Flamingo
  • Frizzled
  • Lim Kinase
  • Planar cell polarity
  • Twinstar

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Twinstar, the Drosophila homolog of cofilin/ADF, is required for planar cell polarity patterning. / Blair, Adrienne; Tomlinson, Andrew; Pham, Hung; Gunsalus, Kristin C.; Goldberg, Michael L.; Laski, Frank A.

In: Development, Vol. 133, No. 9, 05.2006, p. 1789-1797.

Research output: Contribution to journalArticle

Blair, Adrienne ; Tomlinson, Andrew ; Pham, Hung ; Gunsalus, Kristin C. ; Goldberg, Michael L. ; Laski, Frank A. / Twinstar, the Drosophila homolog of cofilin/ADF, is required for planar cell polarity patterning. In: Development. 2006 ; Vol. 133, No. 9. pp. 1789-1797.
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