Tunable membranes for free-flow zone electrophoresis in PDMS microchip using guided self-assembly of silica microbeads

Rafael Song, Lidan Wu, Steven R. Tannenbaum, John S. Wishnok, Jongyoon Han

Research output: Contribution to journalArticle

Abstract

In this paper, we evaluate the strategy of using self-assembled microbeads to build a robust and tunable membrane for free-flow zone electrophoresis in a PDMS microfluidic chip. To fabricate a porous membrane as a salt bridge for free-flow zone electrophoresis, we used silica or polystyrene microbeads between 3-6 μm in diameter and packed them inside a microchannel. After complete evaporation, we infiltrated the porous microbead structure with a positively or negatively charged hydrogel to modify its surface charge polarity. Using this device, we demonstrated binary sorting (separation of positive and negative species at a given pH) of peptides and dyes in standard buffer systems without using sheath flows. The sample loss during sorting could be minimized by using ion selectivity of hydrogel-infiltrated microbead membranes. Our fabrication method enables building a robust membrane for pressure-driven free-flow zone electrophoresis with tunable pore size as well as surface charge polarity.

Original languageEnglish (US)
Pages (from-to)11695-11699
Number of pages5
JournalAnalytical Chemistry
Volume85
Issue number24
DOIs
StatePublished - Dec 17 2013

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Electrophoresis
Silicon Dioxide
Self assembly
Membranes
Hydrogel
Surface charge
Sorting
Polystyrenes
Microchannels
Microfluidics
Pore size
Buffers
Evaporation
Coloring Agents
Salts
Ions
Fabrication
Peptides

ASJC Scopus subject areas

  • Analytical Chemistry

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Tunable membranes for free-flow zone electrophoresis in PDMS microchip using guided self-assembly of silica microbeads. / Song, Rafael; Wu, Lidan; Tannenbaum, Steven R.; Wishnok, John S.; Han, Jongyoon.

In: Analytical Chemistry, Vol. 85, No. 24, 17.12.2013, p. 11695-11699.

Research output: Contribution to journalArticle

Song, Rafael ; Wu, Lidan ; Tannenbaum, Steven R. ; Wishnok, John S. ; Han, Jongyoon. / Tunable membranes for free-flow zone electrophoresis in PDMS microchip using guided self-assembly of silica microbeads. In: Analytical Chemistry. 2013 ; Vol. 85, No. 24. pp. 11695-11699.
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