Tumor necrosis factor alpha secreted from oral squamous cell carcinoma contributes to cancer pain and associated inflammation

Nicole N. Scheff, Yi Ye, Aditi Bhattacharya, Justin MacRae, Dustin N. Hickman, Atul K. Sharma, John C. Dolan, Brian Schmidt

Research output: Contribution to journalArticle

Abstract

Patients with oral cancer report severe pain during function. Inflammation plays a role in the oral cancer microenvironment; however, the role of immune cells and associated secretion of inflammatory mediators in oral cancer pain has not been well defined. In this study, we used 2 oral cancer mouse models: A cell line supernatant injection model and the 4-nitroquinoline-1-oxide (4NQO) chemical carcinogenesis model. We used the 2 models to study changes in immune cell infiltrate and orofacial nociception associated with oral squamous cell carcinoma (oSCC). Oral cancer cell line supernatant inoculation and 4NQO-induced oSCC resulted in functional allodynia and neuronal sensitization of trigeminal tongue afferent neurons. Although the infiltration of immune cells is a prominent component of both oral cancer models, our use of immune-deficient mice demonstrated that oral cancer-induced nociception was not dependent on the inflammatory component. Furthermore, the inflammatory cytokine, tumor necrosis factor alpha (TNFα), was identified in high concentration in oral cancer cell line supernatant and in the tongue tissue of 4NQO-treated mice with oSCC. Inhibition of TNFα signaling abolished oral cancer cell line supernatant-evoked functional allodynia and disrupted T-cell infiltration. With these data, we identified TNFα as a prominent mediator in oral cancer-induced nociception and inflammation, highlighting the need for further investigation in neural-immune communication in cancer pain.

Original languageEnglish (US)
Pages (from-to)2396-2409
Number of pages14
JournalPain
Volume158
Issue number12
DOIs
StatePublished - Jan 1 2017

Fingerprint

Mouth Neoplasms
Squamous Cell Carcinoma
Tumor Necrosis Factor-alpha
Inflammation
4-Nitroquinoline-1-oxide
Nociception
Cell Line
Hyperalgesia
Tongue
Cancer Pain
Chemical Models
Afferent Neurons
Tumor Microenvironment
Carcinogenesis
Communication
Cytokines
T-Lymphocytes
Pain
Injections

Keywords

  • Cancer pain
  • Inflammation
  • Oral squamous cell carcinoma
  • Tumor necrosis factor alpha

ASJC Scopus subject areas

  • Neurology
  • Clinical Neurology
  • Anesthesiology and Pain Medicine

Cite this

Tumor necrosis factor alpha secreted from oral squamous cell carcinoma contributes to cancer pain and associated inflammation. / Scheff, Nicole N.; Ye, Yi; Bhattacharya, Aditi; MacRae, Justin; Hickman, Dustin N.; Sharma, Atul K.; Dolan, John C.; Schmidt, Brian.

In: Pain, Vol. 158, No. 12, 01.01.2017, p. 2396-2409.

Research output: Contribution to journalArticle

Scheff, Nicole N. ; Ye, Yi ; Bhattacharya, Aditi ; MacRae, Justin ; Hickman, Dustin N. ; Sharma, Atul K. ; Dolan, John C. ; Schmidt, Brian. / Tumor necrosis factor alpha secreted from oral squamous cell carcinoma contributes to cancer pain and associated inflammation. In: Pain. 2017 ; Vol. 158, No. 12. pp. 2396-2409.
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