Tropheryma whipplei colonization in HIVinfected individuals is not associated with lung function or inflammation

Shulin Qin, Emily Clausen, Seyed Mehdi Nouraie, Lawrence Kingsley, Deborah McMahon, Eric Kleerup, Laurence Huang, Elodie Ghedin, Ruth M. Greenblatt, Alison Morris

Research output: Contribution to journalArticle

Abstract

Studies demonstrate that Tropheryma whipplei (T. whipplei) is present in the lungs of healthy individuals without acute respiratory symptoms or acute respiratory infection and is more common in the lungs of HIV-infected individuals and in smokers. The impact of T. whipplei colonization in the lung on local inflammation and pulmonary dysfunction in HIVinfected individuals is currently unknown. In this study, we performed specific polymerase chain reaction (PCR) and sequencing for T. whipplei in bronchoalveolar lavage (BAL) and induced sputum (IS) samples in 76 HIV-infected participants from three clinical sites. Pulmonary function and proinflammatory cytokine and chemokine levels in BAL were measured. Frequency of T. whipplei in either BAL or IS was 43.4%. The sensitivity and specificity of IS compared to BAL for detection of T. whipplei was 92.3% and 84.2%, respectively, and isolates of T. whipplei in the BAL and IS in the same subject shared genetic identity. Pulmonary function measures were not associated with T. whipplei colonization, and proinflammatory cytokine and chemokine levels in BAL and plasma as well as percentages of inflammatory cells in BAL and IS were not higher in colonized individuals. Overall, these results indicate that T. whipplei colonization in the lung is common, but may not be associated with decreased pulmonary function or inflammation in HIV-infected individuals.

Original languageEnglish (US)
Article numbere0205065
JournalPLoS One
Volume13
Issue number10
DOIs
StatePublished - Oct 1 2018

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Tropheryma
lung function
Bronchoalveolar Lavage
inflammation
Inflammation
Chemokines
Sputum
Lung
lungs
Cytokines
Polymerase chain reaction
HIV
chemokines
Plasmas
Pneumonia
cytokines
Pedigree
Respiratory Tract Infections
signs and symptoms (animals and humans)
polymerase chain reaction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Qin, S., Clausen, E., Nouraie, S. M., Kingsley, L., McMahon, D., Kleerup, E., ... Morris, A. (2018). Tropheryma whipplei colonization in HIVinfected individuals is not associated with lung function or inflammation. PLoS One, 13(10), [e0205065]. https://doi.org/10.1371/journal.pone.0205065

Tropheryma whipplei colonization in HIVinfected individuals is not associated with lung function or inflammation. / Qin, Shulin; Clausen, Emily; Nouraie, Seyed Mehdi; Kingsley, Lawrence; McMahon, Deborah; Kleerup, Eric; Huang, Laurence; Ghedin, Elodie; Greenblatt, Ruth M.; Morris, Alison.

In: PLoS One, Vol. 13, No. 10, e0205065, 01.10.2018.

Research output: Contribution to journalArticle

Qin, S, Clausen, E, Nouraie, SM, Kingsley, L, McMahon, D, Kleerup, E, Huang, L, Ghedin, E, Greenblatt, RM & Morris, A 2018, 'Tropheryma whipplei colonization in HIVinfected individuals is not associated with lung function or inflammation', PLoS One, vol. 13, no. 10, e0205065. https://doi.org/10.1371/journal.pone.0205065
Qin, Shulin ; Clausen, Emily ; Nouraie, Seyed Mehdi ; Kingsley, Lawrence ; McMahon, Deborah ; Kleerup, Eric ; Huang, Laurence ; Ghedin, Elodie ; Greenblatt, Ruth M. ; Morris, Alison. / Tropheryma whipplei colonization in HIVinfected individuals is not associated with lung function or inflammation. In: PLoS One. 2018 ; Vol. 13, No. 10.
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