Trapping a folding intermediate of the α-helix: Stabilization of the π-helix

Ross Chapman, John L. Kulp, Anupam Patgiri, Neville R. Kallenbach, Clay Bracken, Paramjit Arora

Research output: Contribution to journalArticle

Abstract

We report the design, synthesis, and characterization of a short peptide trapped in a π-helix configuration. This high-energy conformation was nucleated by a preorganized π-turn, which was obtained by replacing an N-terminal intramolecular main chain i and i + 5 hydrogen bond with a carbon-carbon bond. Our studies highlight the nucleation parameter as a key factor contributing to the relative instability of the π-helix and allow us to estimate fundamental helix-coil transition parameters for this conformation.

Original languageEnglish (US)
Pages (from-to)4189-4195
Number of pages7
JournalBiochemistry
Volume47
Issue number14
DOIs
StatePublished - Apr 8 2008

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Conformations
Carbon
Stabilization
Hydrogen
Hydrogen bonds
Nucleation
Peptides

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chapman, R., Kulp, J. L., Patgiri, A., Kallenbach, N. R., Bracken, C., & Arora, P. (2008). Trapping a folding intermediate of the α-helix: Stabilization of the π-helix. Biochemistry, 47(14), 4189-4195. https://doi.org/10.1021/bi800136m

Trapping a folding intermediate of the α-helix : Stabilization of the π-helix. / Chapman, Ross; Kulp, John L.; Patgiri, Anupam; Kallenbach, Neville R.; Bracken, Clay; Arora, Paramjit.

In: Biochemistry, Vol. 47, No. 14, 08.04.2008, p. 4189-4195.

Research output: Contribution to journalArticle

Chapman, R, Kulp, JL, Patgiri, A, Kallenbach, NR, Bracken, C & Arora, P 2008, 'Trapping a folding intermediate of the α-helix: Stabilization of the π-helix', Biochemistry, vol. 47, no. 14, pp. 4189-4195. https://doi.org/10.1021/bi800136m
Chapman R, Kulp JL, Patgiri A, Kallenbach NR, Bracken C, Arora P. Trapping a folding intermediate of the α-helix: Stabilization of the π-helix. Biochemistry. 2008 Apr 8;47(14):4189-4195. https://doi.org/10.1021/bi800136m
Chapman, Ross ; Kulp, John L. ; Patgiri, Anupam ; Kallenbach, Neville R. ; Bracken, Clay ; Arora, Paramjit. / Trapping a folding intermediate of the α-helix : Stabilization of the π-helix. In: Biochemistry. 2008 ; Vol. 47, No. 14. pp. 4189-4195.
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