Transgene coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila

Mark Siegal, Daniel L. Hartl

Research output: Contribution to journalArticle

Abstract

Studies of gene function and regulation in transgenic Drosophila are often compromised by the possibility of genomic position effects of gene expression. We have developed a method, called transgene coplacement, in which any two sequences can be positioned at exactly the same site and orientation in the genome. Transgene coplacement makes use of the bacteriophage P1 system of Cre/loxP site-specific recombination, which we have introduced into Drosophila. In the presence of a cre transgene driven by a dual hsp70-Mos1 promoter, a while reporter gene flanked by loxP sites is excised with virtually 100% efficiency both in somatic cells and in germ cells. A strong maternal effect, resulting from Cre recombinase present in the oocyte, is observed as white or mosaic eye color in F1 progeny. Excision in germ cells of the F1 yields a strong grand-maternal effect, observed as a highly skewed ratio of eye-color phenotypes in the F2 generation. The excision reactions of Cre/loxP and the related FLP/FRT system are used to create Drosophila lines in which transgenes are at exactly allelic sites in homologous chromosomes.

Original languageEnglish (US)
Pages (from-to)715-726
Number of pages12
JournalGenetics
Volume144
Issue number2
StatePublished - 1996

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Transgenes
Genetic Recombination
Drosophila
Eye Color
Germ Cells
Bacteriophage P1
Reporter Genes
Oocytes
Chromosomes
Genome
Phenotype
Gene Expression
Genes
Maternal Inheritance

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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Transgene coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila. / Siegal, Mark; Hartl, Daniel L.

In: Genetics, Vol. 144, No. 2, 1996, p. 715-726.

Research output: Contribution to journalArticle

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