Transforming growth factor-β3 therapy delays Postoperative reossification and improves craniofacial growth in craniosynostotic rabbits

J. Gilbert, M. Karski, T. D. Smith, A. M. Burrows, C. Norbutt, M. I. Siegel, B. J. Costello, J. J. Cray, J. E. Losee, Amr Moursi, G. M. Cooper, M. P. Mooney

Research output: Contribution to journalArticle

Abstract

Postoperative reossification is a common clinical correlate following surgery. It has been suggested that an underexpression of transforming growth factor-β3 (TGF-β3) may be related to craniosynostosis and postoperative reossification. Adding TGF-β3 may delay reossification and improve postoperative growth. The present study was designed to test this hypothesis. Thirty 10-day-old New Zealand white rabbits with hereditary coronal suture synostosis were divided into three groups: (1) suturectomy controls (n = 14), (2) suturectomy treated with bovine serum albumin (n = 8), and (3) suturectomy treated with TGF-β3 protein (n = 8). At 10 days of age, a 3-mm × 15-mm coronal suturectomy was performed, and serial three-dimensional (3D) computed tomography (CT) scans and cephalographs were taken at 10, 25, 42, and 84 days of age. Calvaria were harvested at 84 days of age for histomorphometric analysis. Mean differences were analyzed using a group by age analysis of variance. Analysis of the 3D CT scan data revealed that sites treated with TGF-β3 had significantly (P

Original languageEnglish (US)
Pages (from-to)210-221
Number of pages12
JournalCleft Palate-Craniofacial Journal
Volume53
Issue number2
DOIs
StatePublished - Mar 1 2016

Fingerprint

Transforming Growth Factors
Rabbits
Growth
Tomography
Synostosis
Craniosynostoses
Therapeutics
Bovine Serum Albumin
Skull
Sutures
Analysis of Variance
Age Groups
Proteins

Keywords

  • Craniosynostosis
  • Protein therapy
  • Reossification
  • Surgical model
  • Transforming growth factor

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Oral Surgery

Cite this

Transforming growth factor-β3 therapy delays Postoperative reossification and improves craniofacial growth in craniosynostotic rabbits. / Gilbert, J.; Karski, M.; Smith, T. D.; Burrows, A. M.; Norbutt, C.; Siegel, M. I.; Costello, B. J.; Cray, J. J.; Losee, J. E.; Moursi, Amr; Cooper, G. M.; Mooney, M. P.

In: Cleft Palate-Craniofacial Journal, Vol. 53, No. 2, 01.03.2016, p. 210-221.

Research output: Contribution to journalArticle

Gilbert, J, Karski, M, Smith, TD, Burrows, AM, Norbutt, C, Siegel, MI, Costello, BJ, Cray, JJ, Losee, JE, Moursi, A, Cooper, GM & Mooney, MP 2016, 'Transforming growth factor-β3 therapy delays Postoperative reossification and improves craniofacial growth in craniosynostotic rabbits', Cleft Palate-Craniofacial Journal, vol. 53, no. 2, pp. 210-221. https://doi.org/10.1597/14-298
Gilbert, J. ; Karski, M. ; Smith, T. D. ; Burrows, A. M. ; Norbutt, C. ; Siegel, M. I. ; Costello, B. J. ; Cray, J. J. ; Losee, J. E. ; Moursi, Amr ; Cooper, G. M. ; Mooney, M. P. / Transforming growth factor-β3 therapy delays Postoperative reossification and improves craniofacial growth in craniosynostotic rabbits. In: Cleft Palate-Craniofacial Journal. 2016 ; Vol. 53, No. 2. pp. 210-221.
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