Transformation of rabbit arterial smooth muscle cells with Simian virus 40

M. Nachtigal, P. Greenspan, Louis Terracio, S. D. Fowler

Research output: Contribution to journalArticle

Abstract

In vitro studies were carried out to induce viral transformation of vascular smooth muscle cells. Cultured rabbit arterial smooth muscle cells were infected with simian virus 40 (SV 40), and transformed cultures were produced that exhibit altered morphology, increased growth rate and plating efficiency, growth on semi-solid substrate, and chromosomal abnormalities. Nuclear SV 40 T-antigen was detected in all cells of these cultures. Muscle-specific actin was identified by a specific monoclonal antibody suggesting retention of smooth muscle cell characteristics by the transformed cells. Significant cytoplasmic lipid accumulation occurred in transformed cells incubated with β-very low density lipoprotein, as revealed both by chemical analyses and Nile Red lipid staining of the cultures. The transformed smooth muscle cells grow permanently in cell culture. Our investigations show that arterial smooth muscle cells transformed with SV 40 virus exhibit altered phenotypic properties distinct from that of normal arterial smooth muscle cells.

Original languageEnglish (US)
Pages (from-to)225-235
Number of pages11
JournalArchives of Virology
Volume95
Issue number3-4
DOIs
StatePublished - Sep 1987

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Simian virus 40
Smooth Muscle Myocytes
Rabbits
Cell Culture Techniques
Lipids
VLDL Lipoproteins
Viral Tumor Antigens
Growth
Vascular Smooth Muscle
Chromosome Aberrations
Actins
Monoclonal Antibodies
Staining and Labeling
Viruses
Muscles

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Genetics

Cite this

Transformation of rabbit arterial smooth muscle cells with Simian virus 40. / Nachtigal, M.; Greenspan, P.; Terracio, Louis; Fowler, S. D.

In: Archives of Virology, Vol. 95, No. 3-4, 09.1987, p. 225-235.

Research output: Contribution to journalArticle

Nachtigal, M. ; Greenspan, P. ; Terracio, Louis ; Fowler, S. D. / Transformation of rabbit arterial smooth muscle cells with Simian virus 40. In: Archives of Virology. 1987 ; Vol. 95, No. 3-4. pp. 225-235.
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