Transfer of inter-α-inhibitor heavy chains to hyaluronan by surface-linked hyaluronan-TSG-6 complexes

Elisa Colón, Anastasia Shytuhina, Mary Cowman, Philip A. Band, Kristian W. Sanggaard, Jan J. Enghild, Hans Georg Wisniewski

Research output: Contribution to journalArticle

Abstract

Inter-α-inhibitor, TSG-6, and hyaluronan have important functions in fertility and inflammation. Two subunits of inter-α-inhibitor, the heavy chains, form covalent bonds with TSG-6 or hyaluronan in vitro. TSG-6-heavy chain complexes serve as intermediates in the transfer of heavy chains from inter-α-inhibitor to hyaluronan. In vivo, in addition to these complexes, stable ternary complexes of hyaluronan with both TSG-6 and heavy chains have been demonstrated in the ovulatory cumulus oophorus. In our ongoing efforts to characterize the multiple interactions between hyaluronan, TSG-6 and inter-α-inhibitor, we recently characterized the formation of highly stable complexes of TSG-6 with hyaluronan that had been tethered to a solid surface. Here we show that these hyaluronan-TSG-6 complexes are functionally active and transfer heavy chain subunits from inter-α-inhibitor to either free or surface-bound hyaluronan. Transitional hyaluronan-TSG-6-heavy chain complexes do not accumulate in vitro. Our data show the capability for heavy chain transfer by both free TSG-6 and preformed hyaluronan-TSG-6 complexes, suggesting that both might contribute to hyaluronan modification in vivo. Transfer of heavy chains to surface-tethered hyaluronan by either free TSG-6 or surface-tethered hyaluronan-TSG-6 complexes did not affect the CD 44-mediated binding of BW 5147 cells in vitro. We show how TSG-6 and hyaluronan together can deplete inter-α-inhibitor and generate bikunin, as has been observed in sepsis, and discuss the role of TSG-6 in the generation of hyaluronan-heavy chain complexes associated with ovulation, arthritis, and sepsis.

Original languageEnglish (US)
Pages (from-to)2320-2331
Number of pages12
JournalJournal of Biological Chemistry
Volume284
Issue number4
DOIs
StatePublished - Jan 23 2009

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Hyaluronic Acid
Sepsis
Covalent bonds
Ovulation
Arthritis
Fertility

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Colón, E., Shytuhina, A., Cowman, M., Band, P. A., Sanggaard, K. W., Enghild, J. J., & Wisniewski, H. G. (2009). Transfer of inter-α-inhibitor heavy chains to hyaluronan by surface-linked hyaluronan-TSG-6 complexes. Journal of Biological Chemistry, 284(4), 2320-2331. https://doi.org/10.1074/jbc.M807183200

Transfer of inter-α-inhibitor heavy chains to hyaluronan by surface-linked hyaluronan-TSG-6 complexes. / Colón, Elisa; Shytuhina, Anastasia; Cowman, Mary; Band, Philip A.; Sanggaard, Kristian W.; Enghild, Jan J.; Wisniewski, Hans Georg.

In: Journal of Biological Chemistry, Vol. 284, No. 4, 23.01.2009, p. 2320-2331.

Research output: Contribution to journalArticle

Colón, E, Shytuhina, A, Cowman, M, Band, PA, Sanggaard, KW, Enghild, JJ & Wisniewski, HG 2009, 'Transfer of inter-α-inhibitor heavy chains to hyaluronan by surface-linked hyaluronan-TSG-6 complexes', Journal of Biological Chemistry, vol. 284, no. 4, pp. 2320-2331. https://doi.org/10.1074/jbc.M807183200
Colón, Elisa ; Shytuhina, Anastasia ; Cowman, Mary ; Band, Philip A. ; Sanggaard, Kristian W. ; Enghild, Jan J. ; Wisniewski, Hans Georg. / Transfer of inter-α-inhibitor heavy chains to hyaluronan by surface-linked hyaluronan-TSG-6 complexes. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 4. pp. 2320-2331.
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AU - Band, Philip A.

AU - Sanggaard, Kristian W.

AU - Enghild, Jan J.

AU - Wisniewski, Hans Georg

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