Transcriptional regulation of the Drosophila gene zen by competing Smad and Brinker inputs

Christine Rushlow, Pamela F. Colosimo, Meng Chi Lin, Mu Xu, Nikolai Kirov

Research output: Contribution to journalArticle

Abstract

The establishment of expression domains of developmentally regulated genes depends on cues provided by different concentrations of transcriptional activators and repressors. Here we analyze the regulation of the Drosophila gene zen, which is a target of the Decapentaplegic (Dpp) signaling pathway during cellular blastoderm formation. We show that low levels of the Dpp signal transducer p-Mad (phosphorylated Mad), together with the recently discovered negative regulator Brinker (Brk), define the spatial limits of zen transcription in a broad dorsal-on/ventral-off domain. The subsequent refinement of this pattern to the dorsal-most cells, however, correlates with high levels of p-Mad that accumulate in the same region during late blastoderm. Examination of the zen regulatory sequences revealed the presence of multiple Mad and Brk binding sites, and our results indicate that a full occupancy of the Mad sites due to high concentrations of nuclear Mad is the primary mechanism for refinement of zen. Interestingly, several Mad and Brk binding sites overlap, and we show that Mad and Brk cannot bind simultaneously to such sites. We propose a model whereby competition between Mad and Brk determines spatially restricted domains of expression of Dpp target genes.

Original languageEnglish (US)
Pages (from-to)340-351
Number of pages12
JournalGenes & development
Volume15
Issue number3
DOIs
StatePublished - Feb 1 2001

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Blastoderm
Drosophila
Binding Sites
Genes
Transducers
Cues

Keywords

  • Brk repression
  • Dpp morphogen
  • Smad activation
  • Target genes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Transcriptional regulation of the Drosophila gene zen by competing Smad and Brinker inputs. / Rushlow, Christine; Colosimo, Pamela F.; Lin, Meng Chi; Xu, Mu; Kirov, Nikolai.

In: Genes & development, Vol. 15, No. 3, 01.02.2001, p. 340-351.

Research output: Contribution to journalArticle

Rushlow, Christine ; Colosimo, Pamela F. ; Lin, Meng Chi ; Xu, Mu ; Kirov, Nikolai. / Transcriptional regulation of the Drosophila gene zen by competing Smad and Brinker inputs. In: Genes & development. 2001 ; Vol. 15, No. 3. pp. 340-351.
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