Transcriptional profile of the arabidopsis root quiescent center

Tal Nawy, Ji Young Lee, Juliette Colinas, Jean Y. Wang, Sumena C. Thongrod, Jocelyn E. Malamy, Kenneth Birnbaum, Philip N. Benfey

Research output: Contribution to journalArticle

Abstract

The self-renewal characteristics of stem cells render them vital engines of development. To better understand the molecular mechanisms that determine the properties of stem cells, transcript profiling was conducted on quiescent center (QC) cells from the Arabidopsis thaliana root meristem. The AGAMOUS-LIKE 42 (AGL42) gene, which encodes a MADS box transcription factor whose expression is enriched in the QC, was used to mark these cells. RNA was isolated from sorted cells, labeled, and hybridized to Affymetrix microarrays. Comparisons with digital in situ expression profiles of surrounding tissues identified a set of genes enriched in the QC. Promoter regions from a subset of transcription factors identified as enriched in the QC conferred expression in the QC. These studies demonstrated that it is possible to successfully isolate and profile a rare cell type in the plant. Mutations in all enriched transcription factor genes including AGL42 exhibited no detectable root phenotype, raising the possibility of a high degree of functional redundancy in the QC.

Original languageEnglish (US)
Pages (from-to)1908-1925
Number of pages18
JournalPlant Cell
Volume17
Issue number7
DOIs
StatePublished - 2005

Fingerprint

Arabidopsis
Transcription Factors
Genes
Stem cells
transcription factors
stem cells
Meristem
Plant Cells
cells
Microarrays
Genetic Promoter Regions
Redundancy
genes
root meristems
Stem Cells
engines
RNA
Tissue
Engines
Phenotype

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Nawy, T., Lee, J. Y., Colinas, J., Wang, J. Y., Thongrod, S. C., Malamy, J. E., ... Benfey, P. N. (2005). Transcriptional profile of the arabidopsis root quiescent center. Plant Cell, 17(7), 1908-1925. https://doi.org/10.1105/tpc.105.031724

Transcriptional profile of the arabidopsis root quiescent center. / Nawy, Tal; Lee, Ji Young; Colinas, Juliette; Wang, Jean Y.; Thongrod, Sumena C.; Malamy, Jocelyn E.; Birnbaum, Kenneth; Benfey, Philip N.

In: Plant Cell, Vol. 17, No. 7, 2005, p. 1908-1925.

Research output: Contribution to journalArticle

Nawy, T, Lee, JY, Colinas, J, Wang, JY, Thongrod, SC, Malamy, JE, Birnbaum, K & Benfey, PN 2005, 'Transcriptional profile of the arabidopsis root quiescent center', Plant Cell, vol. 17, no. 7, pp. 1908-1925. https://doi.org/10.1105/tpc.105.031724
Nawy T, Lee JY, Colinas J, Wang JY, Thongrod SC, Malamy JE et al. Transcriptional profile of the arabidopsis root quiescent center. Plant Cell. 2005;17(7):1908-1925. https://doi.org/10.1105/tpc.105.031724
Nawy, Tal ; Lee, Ji Young ; Colinas, Juliette ; Wang, Jean Y. ; Thongrod, Sumena C. ; Malamy, Jocelyn E. ; Birnbaum, Kenneth ; Benfey, Philip N. / Transcriptional profile of the arabidopsis root quiescent center. In: Plant Cell. 2005 ; Vol. 17, No. 7. pp. 1908-1925.
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