Transcription dynamically patterns the meiotic chromosome-axis interface

X. Sun, L. Huang, T. E. Markowitz, H. G. Blitzblau, D. Chen, F. Klein, A. Hochwagen

Research output: Contribution to journalArticle

Abstract

Meiotic chromosomes are highly compacted yet remain transcriptionally active. To understand how chromosome folding accommodates transcription, we investigated the assembly of the axial element, the proteinaceous structure that compacts meiotic chromosomes and promotes recombination and fertility. We found that the axial element proteins of budding yeast are flexibly anchored to chromatin by the ring-like cohesin complex. The ubiquitous presence of cohesin at sites of convergent transcription provides well-dispersed points for axis attachment and thus chromosome compaction. Axis protein enrichment at these sites directly correlates with the propensity for recombination initiation nearby. A separate modulating mechanism that requires the conserved axial-element component Hop1 biases axis protein binding towards small chromosomes. Importantly, axis anchoring by cohesin is adjustable and readily displaced in the direction of transcription by the transcriptional machinery. We propose that such robust but flexible tethering allows the axial element to promote recombination while easily adapting to changes in chromosome activity.
Original languageUndefined
JournaleLife
Volume4
DOIs
StatePublished - 2015

Cite this

Sun, X., Huang, L., Markowitz, T. E., Blitzblau, H. G., Chen, D., Klein, F., & Hochwagen, A. (2015). Transcription dynamically patterns the meiotic chromosome-axis interface. eLife, 4. https://doi.org/10.7554/eLife.07424

Transcription dynamically patterns the meiotic chromosome-axis interface. / Sun, X.; Huang, L.; Markowitz, T. E.; Blitzblau, H. G.; Chen, D.; Klein, F.; Hochwagen, A.

In: eLife, Vol. 4, 2015.

Research output: Contribution to journalArticle

Sun, X, Huang, L, Markowitz, TE, Blitzblau, HG, Chen, D, Klein, F & Hochwagen, A 2015, 'Transcription dynamically patterns the meiotic chromosome-axis interface', eLife, vol. 4. https://doi.org/10.7554/eLife.07424
Sun X, Huang L, Markowitz TE, Blitzblau HG, Chen D, Klein F et al. Transcription dynamically patterns the meiotic chromosome-axis interface. eLife. 2015;4. https://doi.org/10.7554/eLife.07424
Sun, X. ; Huang, L. ; Markowitz, T. E. ; Blitzblau, H. G. ; Chen, D. ; Klein, F. ; Hochwagen, A. / Transcription dynamically patterns the meiotic chromosome-axis interface. In: eLife. 2015 ; Vol. 4.
@article{41e3346c49204e85acdba1345acf6d37,
title = "Transcription dynamically patterns the meiotic chromosome-axis interface",
abstract = "Meiotic chromosomes are highly compacted yet remain transcriptionally active. To understand how chromosome folding accommodates transcription, we investigated the assembly of the axial element, the proteinaceous structure that compacts meiotic chromosomes and promotes recombination and fertility. We found that the axial element proteins of budding yeast are flexibly anchored to chromatin by the ring-like cohesin complex. The ubiquitous presence of cohesin at sites of convergent transcription provides well-dispersed points for axis attachment and thus chromosome compaction. Axis protein enrichment at these sites directly correlates with the propensity for recombination initiation nearby. A separate modulating mechanism that requires the conserved axial-element component Hop1 biases axis protein binding towards small chromosomes. Importantly, axis anchoring by cohesin is adjustable and readily displaced in the direction of transcription by the transcriptional machinery. We propose that such robust but flexible tethering allows the axial element to promote recombination while easily adapting to changes in chromosome activity.",
author = "X. Sun and L. Huang and Markowitz, {T. E.} and Blitzblau, {H. G.} and D. Chen and F. Klein and A. Hochwagen",
note = "2050-084x Sun, Xiaoji Huang, Lingzhi Markowitz, Tovah E Blitzblau, Hannah G Chen, Doris Klein, Franz Hochwagen, Andreas R01 GM088248/GM/NIGMS NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England Elife. 2015 Aug 10;4. doi: 10.7554/eLife.07424.",
year = "2015",
doi = "10.7554/eLife.07424",
language = "Undefined",
volume = "4",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",

}

TY - JOUR

T1 - Transcription dynamically patterns the meiotic chromosome-axis interface

AU - Sun, X.

AU - Huang, L.

AU - Markowitz, T. E.

AU - Blitzblau, H. G.

AU - Chen, D.

AU - Klein, F.

AU - Hochwagen, A.

N1 - 2050-084x Sun, Xiaoji Huang, Lingzhi Markowitz, Tovah E Blitzblau, Hannah G Chen, Doris Klein, Franz Hochwagen, Andreas R01 GM088248/GM/NIGMS NIH HHS/United States Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't England Elife. 2015 Aug 10;4. doi: 10.7554/eLife.07424.

PY - 2015

Y1 - 2015

N2 - Meiotic chromosomes are highly compacted yet remain transcriptionally active. To understand how chromosome folding accommodates transcription, we investigated the assembly of the axial element, the proteinaceous structure that compacts meiotic chromosomes and promotes recombination and fertility. We found that the axial element proteins of budding yeast are flexibly anchored to chromatin by the ring-like cohesin complex. The ubiquitous presence of cohesin at sites of convergent transcription provides well-dispersed points for axis attachment and thus chromosome compaction. Axis protein enrichment at these sites directly correlates with the propensity for recombination initiation nearby. A separate modulating mechanism that requires the conserved axial-element component Hop1 biases axis protein binding towards small chromosomes. Importantly, axis anchoring by cohesin is adjustable and readily displaced in the direction of transcription by the transcriptional machinery. We propose that such robust but flexible tethering allows the axial element to promote recombination while easily adapting to changes in chromosome activity.

AB - Meiotic chromosomes are highly compacted yet remain transcriptionally active. To understand how chromosome folding accommodates transcription, we investigated the assembly of the axial element, the proteinaceous structure that compacts meiotic chromosomes and promotes recombination and fertility. We found that the axial element proteins of budding yeast are flexibly anchored to chromatin by the ring-like cohesin complex. The ubiquitous presence of cohesin at sites of convergent transcription provides well-dispersed points for axis attachment and thus chromosome compaction. Axis protein enrichment at these sites directly correlates with the propensity for recombination initiation nearby. A separate modulating mechanism that requires the conserved axial-element component Hop1 biases axis protein binding towards small chromosomes. Importantly, axis anchoring by cohesin is adjustable and readily displaced in the direction of transcription by the transcriptional machinery. We propose that such robust but flexible tethering allows the axial element to promote recombination while easily adapting to changes in chromosome activity.

U2 - 10.7554/eLife.07424

DO - 10.7554/eLife.07424

M3 - Article

VL - 4

JO - eLife

JF - eLife

SN - 2050-084X

ER -