Transcription and DNA adducts

What happens when the message gets cut off?

David A. Scicchitano, Eugenia C. Olesnicky, Alexandra Dimitri

Research output: Contribution to journalArticle

Abstract

DNA damage located within a gene's transcription unit can cause RNA polymerase to stall at the modified site, resulting in a truncated transcript, or progress past, producing full-length RNA. However, it is not immediately apparent why some lesions pose strong barriers to elongation while others do not. Studies using site-specifically damaged DNA templates have demonstrated that a wide range of lesions can impede the progress of elongating transcription complexes. The collected results of this work provide evidence for the idea that subtle structural elements can influence how an RNA polymerase behaves when it encounters a DNA adduct during elongation. These elements include: (1) the ability of the RNA polymerase active site to accommodate the damaged base; (2) the size and shape of the adduct, which includes the specific modified base; (3) the stereochemistry of the adduct; (4) the base incorporated into the growing transcript; and (5) the local DNA sequence.

Original languageEnglish (US)
Pages (from-to)1537-1548
Number of pages12
JournalDNA Repair
Volume3
Issue number12
DOIs
StatePublished - Dec 2 2004

Fingerprint

DNA Adducts
DNA-Directed RNA Polymerases
Transcription
Elongation
Stereochemistry
DNA sequences
DNA
DNA Damage
Catalytic Domain
Genes
RNA

Keywords

  • DNA damage
  • DNA repair
  • Transcription
  • Transcription-coupled repair

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Transcription and DNA adducts : What happens when the message gets cut off? / Scicchitano, David A.; Olesnicky, Eugenia C.; Dimitri, Alexandra.

In: DNA Repair, Vol. 3, No. 12, 02.12.2004, p. 1537-1548.

Research output: Contribution to journalArticle

Scicchitano, David A. ; Olesnicky, Eugenia C. ; Dimitri, Alexandra. / Transcription and DNA adducts : What happens when the message gets cut off?. In: DNA Repair. 2004 ; Vol. 3, No. 12. pp. 1537-1548.
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