trans-Splicing to Spliceosomal U2 snRNA Suggests Disruption of Branch Site-U2 Pairing during Pre-mRNA Splicing

Duncan Smith, Charles C. Query, Maria M. Konarska

Research output: Contribution to journalArticle

Abstract

Pairing between U2 snRNA and the branch site of spliceosomal introns is essential for spliceosome assembly and is thought to be required for the first catalytic step of splicing. We have identified an RNA comprising the 5′ end of U2 snRNA and the 3′ exon of the ACT1-CUP1 reporter gene, resulting from a trans-splicing reaction in which a 5′ splice site-like sequence in the universally conserved branch site-binding region of U2 is used in trans as a 5′ splice site for both steps of splicing in vivo. Formation of this product occurs in functional spliceosomes assembled on reporter genes whose 5′ splice sites are predicted to bind poorly at the spliceosome catalytic center. Multiple spatially disparate splice sites in U2 can be used, calling into question both the fate of its pairing to the branch site and the details of its role in splicing catalysis.

Original languageEnglish (US)
Pages (from-to)883-890
Number of pages8
JournalMolecular Cell
Volume26
Issue number6
DOIs
StatePublished - Jun 22 2007

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Trans-Splicing
Spliceosomes
RNA Splice Sites
RNA Precursors
Reporter Genes
Catalysis
Introns
Exons
Binding Sites
RNA
U2 small nuclear RNA

Keywords

  • RNA

ASJC Scopus subject areas

  • Molecular Biology

Cite this

trans-Splicing to Spliceosomal U2 snRNA Suggests Disruption of Branch Site-U2 Pairing during Pre-mRNA Splicing. / Smith, Duncan; Query, Charles C.; Konarska, Maria M.

In: Molecular Cell, Vol. 26, No. 6, 22.06.2007, p. 883-890.

Research output: Contribution to journalArticle

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