Towards self-replicating materials of DNA-functionalized colloids

Mirjam E. Leunissen, Rémi Dreyfus, Roujie Sha, Tong Wang, Nadrian C. Seeman, David J. Pine, Paul M. Chaikin

Research output: Contribution to journalArticle

Abstract

We report the first results of ongoing research that involves the creation of a new class of non-biological materials designed to self-replicate and, as a result, to grow exponentially. We propose a system design that exploits the strong specificity and thermal reversibility of the interactions between colloidal particles functionalized with complementary single-stranded DNA 'sticky ends'. Here, we experimentally test the fundamentals of the different steps that constitute the self-replication scheme. First of all, we quantitatively study the equilibrium and kinetic aspects of the aggregation-dissociation behavior of the particles. We find that the dissociation transition is very sharp (∼1 °C) and that it occurs at unexpectedly low temperatures, with the dissociation temperature shifting further down when the fraction of sticky ends becomes smaller. The sharpness of the transition and its sensitivity to the sticky end fraction are important control parameters in our self-replication scheme. We further find that for our present purposes it is best to use a DNA construct with a double-stranded backbone, as this largely prevents unwanted hybridization events, such as secondary structure formation. The latter is seen to lead to peculiar aggregation kinetics, due to a competition between inter- and intra-particle hybridization. Finally, we show how one can obtain dual recognition at different temperatures by functionalizing a single particle species with two distinct DNA sequences and we demonstrate the formation of permanent bonds, using the chemical intercalator psoralen and long-wavelength UV exposure.

Original languageEnglish (US)
Pages (from-to)2422-2430
Number of pages9
JournalSoft Matter
Volume5
Issue number12
DOIs
StatePublished - 2009

Fingerprint

Colloids
colloids
deoxyribonucleic acid
DNA
Agglomeration
Intercalating Agents
Ficusin
dissociation
Kinetics
Single-Stranded DNA
DNA sequences
Temperature
Systems analysis
sharpness
kinetics
Wavelength
systems engineering
temperature
sensitivity
wavelengths

ASJC Scopus subject areas

  • Chemistry(all)
  • Condensed Matter Physics

Cite this

Leunissen, M. E., Dreyfus, R., Sha, R., Wang, T., Seeman, N. C., Pine, D. J., & Chaikin, P. M. (2009). Towards self-replicating materials of DNA-functionalized colloids. Soft Matter, 5(12), 2422-2430. https://doi.org/10.1039/b817679e

Towards self-replicating materials of DNA-functionalized colloids. / Leunissen, Mirjam E.; Dreyfus, Rémi; Sha, Roujie; Wang, Tong; Seeman, Nadrian C.; Pine, David J.; Chaikin, Paul M.

In: Soft Matter, Vol. 5, No. 12, 2009, p. 2422-2430.

Research output: Contribution to journalArticle

Leunissen, ME, Dreyfus, R, Sha, R, Wang, T, Seeman, NC, Pine, DJ & Chaikin, PM 2009, 'Towards self-replicating materials of DNA-functionalized colloids', Soft Matter, vol. 5, no. 12, pp. 2422-2430. https://doi.org/10.1039/b817679e
Leunissen, Mirjam E. ; Dreyfus, Rémi ; Sha, Roujie ; Wang, Tong ; Seeman, Nadrian C. ; Pine, David J. ; Chaikin, Paul M. / Towards self-replicating materials of DNA-functionalized colloids. In: Soft Matter. 2009 ; Vol. 5, No. 12. pp. 2422-2430.
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