Tongue Coating and the Salivary Microbial Communities Vary in Children with Halitosis

Wen Ren, Zhe Xun, Zicheng Wang, Qun Zhang, Xuenan Liu, Hui Zheng, Qian Zhang, Yifei Zhang, Linshuang Zhang, Chunyan Wu, Shuguo Zheng, Nan Qin, S. Dusko Ehrlich, Yihong Li, Xuesong He, Tao Xu, Ting Chen, Feng Chen

Research output: Contribution to journalArticle

Abstract

Halitosis is a common symptom mainly caused by microbial activities in the oral cavity. Here, we used 16S rRNA gene pyrosequencing and metagenomic sequencing to examine oral microbial compositions and their functional variations in children with halitosis. We found that the tongue coating of subjects with halitosis had greater bacterial richness than those of healthy subjects. The relative abundance and prevalence of Leptotrichia wadei and Peptostreptococcus stomatis were higher in tongue coating samples from children with halitosis than those from children without halitosis; Prevotella shahii had higher relative abundance and prevalence in saliva samples from children with halitosis. We present the first comprehensive evaluation of the co-occurrence networks of saliva and tongue coating communities under healthy and halitosis conditions, and investigated patterns of significant differences between these communities. Moreover, we observed that bacterial genes associated with responses to infectious diseases and terpenoid and polyketide metabolism were enriched in subjects with halitosis, but not in healthy subjects. Hydrogen sulphide (H2S)-related metabolic pathways suggested that there was higher microbial production and less usage of H2S in subjects with halitosis. Thus, the mechanism of halitosis was implied for the first time via metagenomic sequencing.

Original languageEnglish (US)
Article number24481
JournalScientific Reports
Volume6
DOIs
StatePublished - Apr 15 2016

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Halitosis
Tongue
Metagenomics
Saliva
Leptotrichia
Healthy Volunteers
Prevotella
Peptostreptococcus
Polyketides
Bacterial Genes
Hydrogen Sulfide
Terpenes
Metabolic Networks and Pathways
rRNA Genes
Communicable Diseases
Mouth

ASJC Scopus subject areas

  • General

Cite this

Ren, W., Xun, Z., Wang, Z., Zhang, Q., Liu, X., Zheng, H., ... Chen, F. (2016). Tongue Coating and the Salivary Microbial Communities Vary in Children with Halitosis. Scientific Reports, 6, [24481]. https://doi.org/10.1038/srep24481

Tongue Coating and the Salivary Microbial Communities Vary in Children with Halitosis. / Ren, Wen; Xun, Zhe; Wang, Zicheng; Zhang, Qun; Liu, Xuenan; Zheng, Hui; Zhang, Qian; Zhang, Yifei; Zhang, Linshuang; Wu, Chunyan; Zheng, Shuguo; Qin, Nan; Ehrlich, S. Dusko; Li, Yihong; He, Xuesong; Xu, Tao; Chen, Ting; Chen, Feng.

In: Scientific Reports, Vol. 6, 24481, 15.04.2016.

Research output: Contribution to journalArticle

Ren, W, Xun, Z, Wang, Z, Zhang, Q, Liu, X, Zheng, H, Zhang, Q, Zhang, Y, Zhang, L, Wu, C, Zheng, S, Qin, N, Ehrlich, SD, Li, Y, He, X, Xu, T, Chen, T & Chen, F 2016, 'Tongue Coating and the Salivary Microbial Communities Vary in Children with Halitosis', Scientific Reports, vol. 6, 24481. https://doi.org/10.1038/srep24481
Ren, Wen ; Xun, Zhe ; Wang, Zicheng ; Zhang, Qun ; Liu, Xuenan ; Zheng, Hui ; Zhang, Qian ; Zhang, Yifei ; Zhang, Linshuang ; Wu, Chunyan ; Zheng, Shuguo ; Qin, Nan ; Ehrlich, S. Dusko ; Li, Yihong ; He, Xuesong ; Xu, Tao ; Chen, Ting ; Chen, Feng. / Tongue Coating and the Salivary Microbial Communities Vary in Children with Halitosis. In: Scientific Reports. 2016 ; Vol. 6.
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