Tissue-specific gene expression profiling by cell sorting

Pui Leng Ip, Kenneth Birnbaum

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Many cell populations have been labeled using stable reporters containing a fluorescent protein. These same marker lines can be used to capture specific cell types or marked cell populations after a brief enzymatic digestion to dissociate tissues. Here, we describe the use of Fluorescence Activated Cell Sorting (FACS) to isolate a rare population of cells marked with Green Fluorescent Protein (GFP). The FACS is a powerful selection tool that can be used to isolate high-quality RNA while selecting for fluorescence, cell size, and other properties.

Original languageEnglish (US)
Title of host publicationPlant Functional Genomics: Methods and Protocols: Second Edition
PublisherSpringer New York
Pages175-183
Number of pages9
ISBN (Print)9781493924448, 9781493924431
DOIs
StatePublished - Mar 11 2015

Fingerprint

Gene Expression Profiling
sorting
gene expression
Flow Cytometry
cells
flow cytometry
Population
Green Fluorescent Proteins
Cell Size
Digestion
green fluorescent protein
Fluorescence
RNA
digestion
tissues
fluorescence
Proteins

Keywords

  • Cell sorting
  • Cell type isolation
  • FACS
  • Plants
  • Quiescent center
  • RNA profiling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Ip, P. L., & Birnbaum, K. (2015). Tissue-specific gene expression profiling by cell sorting. In Plant Functional Genomics: Methods and Protocols: Second Edition (pp. 175-183). Springer New York. https://doi.org/10.1007/978-1-4939-2444-8_8

Tissue-specific gene expression profiling by cell sorting. / Ip, Pui Leng; Birnbaum, Kenneth.

Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, 2015. p. 175-183.

Research output: Chapter in Book/Report/Conference proceedingChapter

Ip, PL & Birnbaum, K 2015, Tissue-specific gene expression profiling by cell sorting. in Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, pp. 175-183. https://doi.org/10.1007/978-1-4939-2444-8_8
Ip PL, Birnbaum K. Tissue-specific gene expression profiling by cell sorting. In Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York. 2015. p. 175-183 https://doi.org/10.1007/978-1-4939-2444-8_8
Ip, Pui Leng ; Birnbaum, Kenneth. / Tissue-specific gene expression profiling by cell sorting. Plant Functional Genomics: Methods and Protocols: Second Edition. Springer New York, 2015. pp. 175-183
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