Abstract
Background Serotonin implication in allergic contact dermatitis was earlier suggested. Objective To study the expression of serotonin (5-HT) and its receptors 1A and 2A during the kinetics of allergic contact dermatitis (ACD). Method Biopsies from 10 female nickel-allergic patients were obtained at 0, 6, 24, 48 and 72 h after nickel application using a patch testing procedure and then analysed by immunohistochemistry. Results Higher 5-HT epidermal immunoreactivity was seen at 0 and 6 h compared with 24, 48 and 72 h. The number of 5-HT labelled platelets was higher at 48 and 72 h compared with 0, 6 and 24 h. There was a decrease in the number of 5-HT1AR positive cells at 24, 48 and 72 h compared with 0 h. Furthermore, epidermal 5-HT1AR immunoreactivity was stronger at 48 and 72 h compared with 0 h. Total (dermis plus epidermis) number of 5-HT2AR positive cells was gradually increased in a time-dependent manner at 6, 24, 48 and 72 h compared with 0 h. Conclusions Our results suggest the implication of 5-HT, 5-HT1AR and 5-HT2AR in the development of human ACD and the possibility to target those receptors at an early stage of this inflammatory condition.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1200-1205 |
| Number of pages | 6 |
| Journal | Journal of the European Academy of Dermatology and Venereology |
| Volume | 25 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 2011 |
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Keywords
- allergic contact dermatitis
- inflammation
- kinetics
- receptor
- serotonin
ASJC Scopus subject areas
- Dermatology
- Infectious Diseases
Cite this
Time-dependent modulation of serotonin and its receptors 1A and 2A expression in allergic contact dermatitis. / Wetterberg, J.; Taher, C.; Azmitia, Efrain; El-Nour, H.
In: Journal of the European Academy of Dermatology and Venereology, Vol. 25, No. 10, 10.2011, p. 1200-1205.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-dependent modulation of serotonin and its receptors 1A and 2A expression in allergic contact dermatitis
AU - Wetterberg, J.
AU - Taher, C.
AU - Azmitia, Efrain
AU - El-Nour, H.
PY - 2011/10
Y1 - 2011/10
N2 - Background Serotonin implication in allergic contact dermatitis was earlier suggested. Objective To study the expression of serotonin (5-HT) and its receptors 1A and 2A during the kinetics of allergic contact dermatitis (ACD). Method Biopsies from 10 female nickel-allergic patients were obtained at 0, 6, 24, 48 and 72 h after nickel application using a patch testing procedure and then analysed by immunohistochemistry. Results Higher 5-HT epidermal immunoreactivity was seen at 0 and 6 h compared with 24, 48 and 72 h. The number of 5-HT labelled platelets was higher at 48 and 72 h compared with 0, 6 and 24 h. There was a decrease in the number of 5-HT1AR positive cells at 24, 48 and 72 h compared with 0 h. Furthermore, epidermal 5-HT1AR immunoreactivity was stronger at 48 and 72 h compared with 0 h. Total (dermis plus epidermis) number of 5-HT2AR positive cells was gradually increased in a time-dependent manner at 6, 24, 48 and 72 h compared with 0 h. Conclusions Our results suggest the implication of 5-HT, 5-HT1AR and 5-HT2AR in the development of human ACD and the possibility to target those receptors at an early stage of this inflammatory condition.
AB - Background Serotonin implication in allergic contact dermatitis was earlier suggested. Objective To study the expression of serotonin (5-HT) and its receptors 1A and 2A during the kinetics of allergic contact dermatitis (ACD). Method Biopsies from 10 female nickel-allergic patients were obtained at 0, 6, 24, 48 and 72 h after nickel application using a patch testing procedure and then analysed by immunohistochemistry. Results Higher 5-HT epidermal immunoreactivity was seen at 0 and 6 h compared with 24, 48 and 72 h. The number of 5-HT labelled platelets was higher at 48 and 72 h compared with 0, 6 and 24 h. There was a decrease in the number of 5-HT1AR positive cells at 24, 48 and 72 h compared with 0 h. Furthermore, epidermal 5-HT1AR immunoreactivity was stronger at 48 and 72 h compared with 0 h. Total (dermis plus epidermis) number of 5-HT2AR positive cells was gradually increased in a time-dependent manner at 6, 24, 48 and 72 h compared with 0 h. Conclusions Our results suggest the implication of 5-HT, 5-HT1AR and 5-HT2AR in the development of human ACD and the possibility to target those receptors at an early stage of this inflammatory condition.
KW - allergic contact dermatitis
KW - inflammation
KW - kinetics
KW - receptor
KW - serotonin
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U2 - 10.1111/j.1468-3083.2010.03952.x
DO - 10.1111/j.1468-3083.2010.03952.x
M3 - Article
VL - 25
SP - 1200
EP - 1205
JO - Journal of the European Academy of Dermatology and Venereology
JF - Journal of the European Academy of Dermatology and Venereology
SN - 0926-9959
IS - 10
ER -