Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23

Sonia Martinez-Caballero, Sergey M. Grigoriev, Johannes M. Herrmann, María Luisa Campo, Kathleen W. Kinnally

Research output: Contribution to journalArticle

Abstract

The TIM23 complex mediates import of preproteins into mitochondria, but little is known of the mechanistic properties of this translocase. Here patch clamping reconstituted inner membranes allowed for first time insights into the structure and function of the preprotein translocase. Our findings indicate that the TIM23 channel has "twin pores" (two equal sized pores that cooperatively gate) thereby strikingly resembling TOM, the translocase of the outer membrane. Tim17p and Tim23p are homologues, but their functions differ. Tim23p acts as receptor for preproteins and may largely constitute the preprotein-conducting passageway. Conversely depletion of Tim17p induces a collapse of the twin pores into a single pore, whereas N terminus deletion or C terminus truncation results in variable sized pores that cooperatively gate. Further analysis of Tim17p mutants indicates that the N terminus is vital for both voltage sensing and protein sorting. These results suggest that although Tim23p is the main structural unit of the pore Tim17p is required for twin pore structure and provides the voltage gate for the TIM23 channel.

Original languageEnglish (US)
Pages (from-to)3584-3593
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number6
DOIs
StatePublished - Jan 9 2007

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Mitochondrial Proteins
Pore structure
Membranes
Mitochondria
Electric potential
Protein Transport
Sorting
Constriction
Proteins
TOM translocase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Martinez-Caballero, S., Grigoriev, S. M., Herrmann, J. M., Campo, M. L., & Kinnally, K. W. (2007). Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. Journal of Biological Chemistry, 282(6), 3584-3593. https://doi.org/10.1074/jbc.M607551200

Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. / Martinez-Caballero, Sonia; Grigoriev, Sergey M.; Herrmann, Johannes M.; Campo, María Luisa; Kinnally, Kathleen W.

In: Journal of Biological Chemistry, Vol. 282, No. 6, 09.01.2007, p. 3584-3593.

Research output: Contribution to journalArticle

Martinez-Caballero, S, Grigoriev, SM, Herrmann, JM, Campo, ML & Kinnally, KW 2007, 'Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23', Journal of Biological Chemistry, vol. 282, no. 6, pp. 3584-3593. https://doi.org/10.1074/jbc.M607551200
Martinez-Caballero, Sonia ; Grigoriev, Sergey M. ; Herrmann, Johannes M. ; Campo, María Luisa ; Kinnally, Kathleen W. / Tim17p regulates the twin pore structure and voltage gating of the mitochondrial protein import complex TIM23. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 6. pp. 3584-3593.
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