Three-dimensional tertiary structure of yeast phenylalanine transfer RNA

S. H. Kim, F. L. Suddath, G. J. Quigley, A. McPherson, J. L. Sussman, A. H J Wang, N. C. Seeman, Alexander Rich

Research output: Contribution to journalArticle

Abstract

The 3-angstrom electron density map of crystalline yeast phenylalanine transfer RNA has provided us with a complete three-dimensional model which defines the positions of all of the nucleotide residues in the molecule. The overall features of the molecule are virtually the same as those seen at a resolution of 4 angstroms except that many additional details of tertiary structure are now visualized. Ten types of hydrogen bonding are identified which define the specificity of tertiary interactions. The molecule is also stabilized by considerable stacking of the planar purities and pyrimidines. This tertiary structure explains, in a simple and direct fashion, chemical modification studies of transfer RNA. Since most of the tertiary interactions involve nucleotides which are common to all transfer RNA's, it is likely that this three-dimensional structure provides a basic pattern of folding which may help to clarify the three-dimensional structure of all transfer RNA's.

Original languageEnglish (US)
Pages (from-to)435-440
Number of pages6
JournalScience
Volume185
Issue number4149
StatePublished - 1974

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Transfer RNA
Phenylalanine
Yeasts
Nucleotides
Pyrimidines
Hydrogen Bonding
Electrons

ASJC Scopus subject areas

  • General

Cite this

Kim, S. H., Suddath, F. L., Quigley, G. J., McPherson, A., Sussman, J. L., Wang, A. H. J., ... Rich, A. (1974). Three-dimensional tertiary structure of yeast phenylalanine transfer RNA. Science, 185(4149), 435-440.

Three-dimensional tertiary structure of yeast phenylalanine transfer RNA. / Kim, S. H.; Suddath, F. L.; Quigley, G. J.; McPherson, A.; Sussman, J. L.; Wang, A. H J; Seeman, N. C.; Rich, Alexander.

In: Science, Vol. 185, No. 4149, 1974, p. 435-440.

Research output: Contribution to journalArticle

Kim, SH, Suddath, FL, Quigley, GJ, McPherson, A, Sussman, JL, Wang, AHJ, Seeman, NC & Rich, A 1974, 'Three-dimensional tertiary structure of yeast phenylalanine transfer RNA', Science, vol. 185, no. 4149, pp. 435-440.
Kim SH, Suddath FL, Quigley GJ, McPherson A, Sussman JL, Wang AHJ et al. Three-dimensional tertiary structure of yeast phenylalanine transfer RNA. Science. 1974;185(4149):435-440.
Kim, S. H. ; Suddath, F. L. ; Quigley, G. J. ; McPherson, A. ; Sussman, J. L. ; Wang, A. H J ; Seeman, N. C. ; Rich, Alexander. / Three-dimensional tertiary structure of yeast phenylalanine transfer RNA. In: Science. 1974 ; Vol. 185, No. 4149. pp. 435-440.
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