Three-arm nucleic acid junctions are flexible

Rong Ine Ma, Neville R. Kallenbach, Richard D. Sheardy, Mary L. Petrillo, Nadrian C. Seeman

Research output: Contribution to journalArticle

Abstract

Nucleic acid junctions are stable analogs of branched DNA structures which occur transiently in living systems. We show here that junctions which contain three double helical arms can be enzymatically oligomerized, using conventional sticky-ended ligation procedures, to create larger complexes. The products consist of a series of linked junctions separated by 20 base pairs. Junction dimers are formed that have free termini only, whereas trimers and larger species are found to be both unclosed and cyclized. The formation of a series of macrocyclic products which, surprisingly, begins with trimers and tetramers indicates that this junction is flexible about a bending axis, and perhaps twist-wise as well. We have obtained the same results from three different 3-arm junctions, two in which the junction is flanked by a 3 Watson-Crick base pairs, and one in which a G-G base pair flanks the junction.

Original languageEnglish (US)
Pages (from-to)9745-9753
Number of pages9
JournalNucleic Acids Research
Volume14
Issue number24
DOIs
StatePublished - Dec 9 1986

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Nucleic acids
Base Pairing
Dimers
Nucleic Acids
DNA
Living Systems
Series
Dimer
Twist
Ligation
Analogue
Living systems

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Ma, R. I., Kallenbach, N. R., Sheardy, R. D., Petrillo, M. L., & Seeman, N. C. (1986). Three-arm nucleic acid junctions are flexible. Nucleic Acids Research, 14(24), 9745-9753. https://doi.org/10.1093/nar/14.24.9745

Three-arm nucleic acid junctions are flexible. / Ma, Rong Ine; Kallenbach, Neville R.; Sheardy, Richard D.; Petrillo, Mary L.; Seeman, Nadrian C.

In: Nucleic Acids Research, Vol. 14, No. 24, 09.12.1986, p. 9745-9753.

Research output: Contribution to journalArticle

Ma, RI, Kallenbach, NR, Sheardy, RD, Petrillo, ML & Seeman, NC 1986, 'Three-arm nucleic acid junctions are flexible', Nucleic Acids Research, vol. 14, no. 24, pp. 9745-9753. https://doi.org/10.1093/nar/14.24.9745
Ma RI, Kallenbach NR, Sheardy RD, Petrillo ML, Seeman NC. Three-arm nucleic acid junctions are flexible. Nucleic Acids Research. 1986 Dec 9;14(24):9745-9753. https://doi.org/10.1093/nar/14.24.9745
Ma, Rong Ine ; Kallenbach, Neville R. ; Sheardy, Richard D. ; Petrillo, Mary L. ; Seeman, Nadrian C. / Three-arm nucleic acid junctions are flexible. In: Nucleic Acids Research. 1986 ; Vol. 14, No. 24. pp. 9745-9753.
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