Thioesterase superfamily member 1 suppresses cold thermogenesis by limiting the oxidation of lipid droplet-derived fatty acids in brown adipose tissue

Kosuke Okada, Katherine B. LeClair, Yongzhao Zhang, Yingxia Li, Cafer Ozdemir, Tibor I. Krisko, Susan J. Hagen, Rebecca Betensky, Alexander S. Banks, David E. Cohen

Research output: Contribution to journalArticle

Abstract

Objective: Non-shivering thermogenesis in brown adipose tissue (BAT) plays a central role in energy homeostasis. Thioesterase superfamily member 1 (Them1), a BAT-enriched long chain fatty acyl-CoA thioesterase, is upregulated by cold and downregulated by warm ambient temperatures. Them1-/- mice exhibit increased energy expenditure and resistance to diet-induced obesity and diabetes, but the mechanistic contribution of Them1 to the regulation of cold thermogenesis remains unknown. Methods: Them1-/- and Them1+/+ mice were subjected to continuous metabolic monitoring to quantify the effects of ambient temperatures ranging from thermoneutrality (30 °C) to cold (4 °C) on energy expenditure, core body temperature, physical activity and food intake. The effects of Them1 expression on O2 consumption rates, thermogenic gene expression and lipolytic protein activation were determined ex vivo in BAT and in primary brown adipocytes. Results: Them1 suppressed thermogenesis in mice even in the setting of ongoing cold exposure. Without affecting thermogenic gene transcription, Them1 reduced O2 consumption rates in both isolated BAT and primary brown adipocytes. This was attributable to decreased mitochondrial oxidation of endogenous but not exogenous fatty acids. Conclusions: These results show that Them1 may act as a break on uncontrolled heat production and limit the extent of energy expenditure. Pharmacologic inhibition of Them1 could provide a targeted strategy for the management of metabolic disorders via activation of brown fat.

Original languageEnglish (US)
Pages (from-to)340-351
Number of pages12
JournalMolecular Metabolism
Volume5
Issue number5
DOIs
StatePublished - May 1 2016

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Brown Adipose Tissue
Thermogenesis
Fatty Acids
Brown Adipocytes
Energy Metabolism
Acyl Coenzyme A
Palmitoyl-CoA Hydrolase
Temperature
Body Temperature
Homeostasis
Down-Regulation
Obesity
Eating
Lipid Droplets
Diet
Gene Expression
Genes
Proteins

Keywords

  • Acyl-CoA thioesterase
  • Energy expenditure
  • Fatty acyl-CoA
  • Mitochondria
  • Obesity

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Thioesterase superfamily member 1 suppresses cold thermogenesis by limiting the oxidation of lipid droplet-derived fatty acids in brown adipose tissue. / Okada, Kosuke; LeClair, Katherine B.; Zhang, Yongzhao; Li, Yingxia; Ozdemir, Cafer; Krisko, Tibor I.; Hagen, Susan J.; Betensky, Rebecca; Banks, Alexander S.; Cohen, David E.

In: Molecular Metabolism, Vol. 5, No. 5, 01.05.2016, p. 340-351.

Research output: Contribution to journalArticle

Okada, Kosuke ; LeClair, Katherine B. ; Zhang, Yongzhao ; Li, Yingxia ; Ozdemir, Cafer ; Krisko, Tibor I. ; Hagen, Susan J. ; Betensky, Rebecca ; Banks, Alexander S. ; Cohen, David E. / Thioesterase superfamily member 1 suppresses cold thermogenesis by limiting the oxidation of lipid droplet-derived fatty acids in brown adipose tissue. In: Molecular Metabolism. 2016 ; Vol. 5, No. 5. pp. 340-351.
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AU - Zhang, Yongzhao

AU - Li, Yingxia

AU - Ozdemir, Cafer

AU - Krisko, Tibor I.

AU - Hagen, Susan J.

AU - Betensky, Rebecca

AU - Banks, Alexander S.

AU - Cohen, David E.

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AB - Objective: Non-shivering thermogenesis in brown adipose tissue (BAT) plays a central role in energy homeostasis. Thioesterase superfamily member 1 (Them1), a BAT-enriched long chain fatty acyl-CoA thioesterase, is upregulated by cold and downregulated by warm ambient temperatures. Them1-/- mice exhibit increased energy expenditure and resistance to diet-induced obesity and diabetes, but the mechanistic contribution of Them1 to the regulation of cold thermogenesis remains unknown. Methods: Them1-/- and Them1+/+ mice were subjected to continuous metabolic monitoring to quantify the effects of ambient temperatures ranging from thermoneutrality (30 °C) to cold (4 °C) on energy expenditure, core body temperature, physical activity and food intake. The effects of Them1 expression on O2 consumption rates, thermogenic gene expression and lipolytic protein activation were determined ex vivo in BAT and in primary brown adipocytes. Results: Them1 suppressed thermogenesis in mice even in the setting of ongoing cold exposure. Without affecting thermogenic gene transcription, Them1 reduced O2 consumption rates in both isolated BAT and primary brown adipocytes. This was attributable to decreased mitochondrial oxidation of endogenous but not exogenous fatty acids. Conclusions: These results show that Them1 may act as a break on uncontrolled heat production and limit the extent of energy expenditure. Pharmacologic inhibition of Them1 could provide a targeted strategy for the management of metabolic disorders via activation of brown fat.

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