Thin layer composite molecularly imprinted membranes for selective separation of cAMP

Nidal Hilal, V. Kochkodan, G. Busca, O. Kochkodan, B. P. Atkin

Research output: Contribution to journalArticle

Abstract

Composite microfiltration membranes imprinted with adenosine 3′:5′-cyclic monophosphate (cAMP) were developed using photoinitiated copolymerisation of a functional monomer (diethylaminoethylmetacrylate (DEAEM), 2-hydroxyethyl methacrylate (HEMA), methacrylic acid (MA) and trimethylopropanethrimethacrylate (TRIM)) as crosslinker in the presence of cAMP as template, followed by a deposition of molecular imprinted polymer layer (MIP) on the surface of polyvinilidene fluoride (PVDF) membranes. As a result, membranes covered with a thin layer of imprinted polymer selective to cAMP were obtained with different degrees of modification. The effects of the type and concentration of the functional monomer, as well as the crosslinker's concentration, on the binding of cAMP on MIP membranes have been evaluated. It was concluded that MIP membranes binding capability is a result of both multi-sites binding to the template via hydrogen bonding and the correct position of functional groups involved in binding in imprinted polymer. Profile imaging mode atomic force microscopy has been used to visualise the surface structure of molecularly imprinted membranes and to quantify pore size and surface characteristics.

Original languageEnglish (US)
Pages (from-to)281-289
Number of pages9
JournalSeparation and Purification Technology
Volume31
Issue number3
DOIs
StatePublished - Jun 1 2003

Fingerprint

Polymers
Membranes
Composite materials
Monomers
Microfiltration
Binding sites
Fluorides
Surface structure
Copolymerization
Functional groups
Pore size
Atomic force microscopy
Hydrogen bonds
Binding Sites
Imaging techniques
Acids

Keywords

  • Molecularly imprinted membranes
  • Pore size
  • Selective separation
  • Surface modification
  • Surface roughness

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

Cite this

Thin layer composite molecularly imprinted membranes for selective separation of cAMP. / Hilal, Nidal; Kochkodan, V.; Busca, G.; Kochkodan, O.; Atkin, B. P.

In: Separation and Purification Technology, Vol. 31, No. 3, 01.06.2003, p. 281-289.

Research output: Contribution to journalArticle

Hilal, Nidal ; Kochkodan, V. ; Busca, G. ; Kochkodan, O. ; Atkin, B. P. / Thin layer composite molecularly imprinted membranes for selective separation of cAMP. In: Separation and Purification Technology. 2003 ; Vol. 31, No. 3. pp. 281-289.
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