Thermochemical scanning probe lithography of protein gradients at the nanoscale

E. Albisetti, K. M. Carroll, X. Lu, J. E. Curtis, D. Petti, R. Bertacco, Elisa Riedo

Research output: Contribution to journalArticle

Abstract

Patterning nanoscale protein gradients is crucial for studying a variety of cellular processes in vitro. Despite the recent development in nano-fabrication technology, combining nanometric resolution and fine control of protein concentrations is still an open challenge. Here, we demonstrate the use of thermochemical scanning probe lithography (tc-SPL) for defining micro- and nano-sized patterns with precisely controlled protein concentration. First, tc-SPL is performed by scanning a heatable atomic force microscopy tip on a polymeric substrate, for locally exposing reactive amino groups on the surface, then the substrate is functionalized with streptavidin and laminin proteins. We show, by fluorescence microscopy on the patterned gradients, that it is possible to precisely tune the concentration of the immobilized proteins by varying the patterning parameters during tc-SPL. This paves the way to the use of tc-SPL for defining protein gradients at the nanoscale, to be used as chemical cues e.g. for studying and regulating cellular processes in vitro.

Original languageEnglish (US)
Article number315302
JournalNanotechnology
Volume27
Issue number31
DOIs
StatePublished - Jun 24 2016

Fingerprint

Lithography
Proteins
Scanning
Immobilized Proteins
Streptavidin
Fluorescence microscopy
Laminin
Substrates
Nanotechnology
Atomic force microscopy

Keywords

  • biofunctionalization
  • extracellular matrix
  • nanopatterning
  • protein gradient
  • scanning probe lithography
  • surface functionalization

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Albisetti, E., Carroll, K. M., Lu, X., Curtis, J. E., Petti, D., Bertacco, R., & Riedo, E. (2016). Thermochemical scanning probe lithography of protein gradients at the nanoscale. Nanotechnology, 27(31), [315302]. https://doi.org/10.1088/0957-4484/27/31/315302

Thermochemical scanning probe lithography of protein gradients at the nanoscale. / Albisetti, E.; Carroll, K. M.; Lu, X.; Curtis, J. E.; Petti, D.; Bertacco, R.; Riedo, Elisa.

In: Nanotechnology, Vol. 27, No. 31, 315302, 24.06.2016.

Research output: Contribution to journalArticle

Albisetti, E, Carroll, KM, Lu, X, Curtis, JE, Petti, D, Bertacco, R & Riedo, E 2016, 'Thermochemical scanning probe lithography of protein gradients at the nanoscale', Nanotechnology, vol. 27, no. 31, 315302. https://doi.org/10.1088/0957-4484/27/31/315302
Albisetti E, Carroll KM, Lu X, Curtis JE, Petti D, Bertacco R et al. Thermochemical scanning probe lithography of protein gradients at the nanoscale. Nanotechnology. 2016 Jun 24;27(31). 315302. https://doi.org/10.1088/0957-4484/27/31/315302
Albisetti, E. ; Carroll, K. M. ; Lu, X. ; Curtis, J. E. ; Petti, D. ; Bertacco, R. ; Riedo, Elisa. / Thermochemical scanning probe lithography of protein gradients at the nanoscale. In: Nanotechnology. 2016 ; Vol. 27, No. 31.
@article{26206b95143c4e0eb2d8e68954cca31d,
title = "Thermochemical scanning probe lithography of protein gradients at the nanoscale",
abstract = "Patterning nanoscale protein gradients is crucial for studying a variety of cellular processes in vitro. Despite the recent development in nano-fabrication technology, combining nanometric resolution and fine control of protein concentrations is still an open challenge. Here, we demonstrate the use of thermochemical scanning probe lithography (tc-SPL) for defining micro- and nano-sized patterns with precisely controlled protein concentration. First, tc-SPL is performed by scanning a heatable atomic force microscopy tip on a polymeric substrate, for locally exposing reactive amino groups on the surface, then the substrate is functionalized with streptavidin and laminin proteins. We show, by fluorescence microscopy on the patterned gradients, that it is possible to precisely tune the concentration of the immobilized proteins by varying the patterning parameters during tc-SPL. This paves the way to the use of tc-SPL for defining protein gradients at the nanoscale, to be used as chemical cues e.g. for studying and regulating cellular processes in vitro.",
keywords = "biofunctionalization, extracellular matrix, nanopatterning, protein gradient, scanning probe lithography, surface functionalization",
author = "E. Albisetti and Carroll, {K. M.} and X. Lu and Curtis, {J. E.} and D. Petti and R. Bertacco and Elisa Riedo",
year = "2016",
month = "6",
day = "24",
doi = "10.1088/0957-4484/27/31/315302",
language = "English (US)",
volume = "27",
journal = "Nanotechnology",
issn = "0957-4484",
publisher = "IOP Publishing Ltd.",
number = "31",

}

TY - JOUR

T1 - Thermochemical scanning probe lithography of protein gradients at the nanoscale

AU - Albisetti, E.

AU - Carroll, K. M.

AU - Lu, X.

AU - Curtis, J. E.

AU - Petti, D.

AU - Bertacco, R.

AU - Riedo, Elisa

PY - 2016/6/24

Y1 - 2016/6/24

N2 - Patterning nanoscale protein gradients is crucial for studying a variety of cellular processes in vitro. Despite the recent development in nano-fabrication technology, combining nanometric resolution and fine control of protein concentrations is still an open challenge. Here, we demonstrate the use of thermochemical scanning probe lithography (tc-SPL) for defining micro- and nano-sized patterns with precisely controlled protein concentration. First, tc-SPL is performed by scanning a heatable atomic force microscopy tip on a polymeric substrate, for locally exposing reactive amino groups on the surface, then the substrate is functionalized with streptavidin and laminin proteins. We show, by fluorescence microscopy on the patterned gradients, that it is possible to precisely tune the concentration of the immobilized proteins by varying the patterning parameters during tc-SPL. This paves the way to the use of tc-SPL for defining protein gradients at the nanoscale, to be used as chemical cues e.g. for studying and regulating cellular processes in vitro.

AB - Patterning nanoscale protein gradients is crucial for studying a variety of cellular processes in vitro. Despite the recent development in nano-fabrication technology, combining nanometric resolution and fine control of protein concentrations is still an open challenge. Here, we demonstrate the use of thermochemical scanning probe lithography (tc-SPL) for defining micro- and nano-sized patterns with precisely controlled protein concentration. First, tc-SPL is performed by scanning a heatable atomic force microscopy tip on a polymeric substrate, for locally exposing reactive amino groups on the surface, then the substrate is functionalized with streptavidin and laminin proteins. We show, by fluorescence microscopy on the patterned gradients, that it is possible to precisely tune the concentration of the immobilized proteins by varying the patterning parameters during tc-SPL. This paves the way to the use of tc-SPL for defining protein gradients at the nanoscale, to be used as chemical cues e.g. for studying and regulating cellular processes in vitro.

KW - biofunctionalization

KW - extracellular matrix

KW - nanopatterning

KW - protein gradient

KW - scanning probe lithography

KW - surface functionalization

UR - http://www.scopus.com/inward/record.url?scp=84978388521&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978388521&partnerID=8YFLogxK

U2 - 10.1088/0957-4484/27/31/315302

DO - 10.1088/0957-4484/27/31/315302

M3 - Article

VL - 27

JO - Nanotechnology

JF - Nanotechnology

SN - 0957-4484

IS - 31

M1 - 315302

ER -