Thermal denaturation of hydrated wool keratin by 1H solid-state NMR

Maria Baias, Dan E. Demco, Crisan Popescu, Radu Fechete, Claudiu Melian, Bernhard Blümich, Martin Möller

Research output: Contribution to journalArticle

Abstract

Thermal denaturation of hydrated keratin in wool was investigated by NMR using 1H wide-line spectra to obtain the phase composition and 1H spin-diffusion experiments using a double-quantum filter to obtain the domain sizes for the wool fibers. The denaturation process detected by DSC takes place for wool fibers in deuterated water in the temperature range 140-144 *C. The phase composition measured by 1H wide line NMR spectra reveals a rigid, semirigid and an amorphous phase for temperatures in the range 25-160 *C. A dramatic change in the phase composition was detected around 142 *C, corresponding to the denaturation temperature. The morphological domain sizes measured by 1H spin-diffusion NMR experiments were obtain from the solutions of the spin-diffusion equations for two-dimensional rectangular and cylindrical morphologies. The keratin mobility gradient in the interfacial region at different denaturation temperatures was measured from the 1H spin-diffusion data. A qualitative model describing the denaturation process of hydrated keratin protein was developed that explains the changes in domain thickness, spin diffusivities, phase composition, and thermodynamic parameters.

Original languageEnglish (US)
Pages (from-to)2184-2192
Number of pages9
JournalJournal of Physical Chemistry B
Volume113
Issue number7
DOIs
StatePublished - Feb 19 2009

Fingerprint

keratins
Keratin
wool
Denaturation
biopolymer denaturation
Keratins
Wool
Phase composition
Nuclear magnetic resonance
solid state
nuclear magnetic resonance
Wool fibers
Temperature
fibers
temperature
diffusivity
line spectra
Experiments
Hot Temperature
Thermodynamics

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Materials Chemistry
  • Surfaces, Coatings and Films

Cite this

Baias, M., Demco, D. E., Popescu, C., Fechete, R., Melian, C., Blümich, B., & Möller, M. (2009). Thermal denaturation of hydrated wool keratin by 1H solid-state NMR. Journal of Physical Chemistry B, 113(7), 2184-2192. https://doi.org/10.1021/jp8094616

Thermal denaturation of hydrated wool keratin by 1H solid-state NMR. / Baias, Maria; Demco, Dan E.; Popescu, Crisan; Fechete, Radu; Melian, Claudiu; Blümich, Bernhard; Möller, Martin.

In: Journal of Physical Chemistry B, Vol. 113, No. 7, 19.02.2009, p. 2184-2192.

Research output: Contribution to journalArticle

Baias, M, Demco, DE, Popescu, C, Fechete, R, Melian, C, Blümich, B & Möller, M 2009, 'Thermal denaturation of hydrated wool keratin by 1H solid-state NMR', Journal of Physical Chemistry B, vol. 113, no. 7, pp. 2184-2192. https://doi.org/10.1021/jp8094616
Baias M, Demco DE, Popescu C, Fechete R, Melian C, Blümich B et al. Thermal denaturation of hydrated wool keratin by 1H solid-state NMR. Journal of Physical Chemistry B. 2009 Feb 19;113(7):2184-2192. https://doi.org/10.1021/jp8094616
Baias, Maria ; Demco, Dan E. ; Popescu, Crisan ; Fechete, Radu ; Melian, Claudiu ; Blümich, Bernhard ; Möller, Martin. / Thermal denaturation of hydrated wool keratin by 1H solid-state NMR. In: Journal of Physical Chemistry B. 2009 ; Vol. 113, No. 7. pp. 2184-2192.
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