The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription

Erica Cavellán, Patrik Asp, Piergiorgio Percipalle, Ann Kristin Östlund Farrants

Research output: Contribution to journalArticle

Abstract

The WSTF (Williams syndrome transcription factor) protein is involved in vitamin D-mediated transcription and replication as a component of two distinct ATP-dependent chromatin remodeling complexes, WINAC and WICH, respectively. We show here that the WICH complex (WSTF-SNF2h) interacts with several nuclear proteins as follows: Sf3b155/SAP155, RNA helicase II/Guα, Myb-binding protein 1a, CSB, the proto-oncogene Dek, and nuclear myosin 1 in a large 3-MDa assembly, B-WICH, during active transcription. B-WICH also contains RNAs, 45 S rRNA, 5 S rRNA, 7SL RNA, and traces of the U2 small nuclear RNA. The core proteins, WSTF, SNF2h, and nuclear myosin 1, are associated with the RNA polymerase III genes 5 S rRNA genes and 7SL, and post-transcriptional silencing of WSTF reduces the levels of these transcripts. Our results show that a WSTF-SNF2h assembly is involved in RNA polymerase III transcription, and we suggest that WSTF-SNF2h-NM1 forms a platform in transcription while providing chromatin remodeling.

Original languageEnglish (US)
Pages (from-to)16264-16271
Number of pages8
JournalJournal of Biological Chemistry
Volume281
Issue number24
DOIs
StatePublished - Jun 16 2006

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Williams Syndrome
Chromatin Assembly and Disassembly
Transcription
Nuclear Proteins
Chromatin
Transcription Factors
RNA Polymerase III
Myosins
Genes
RNA Helicases
Proto-Oncogenes
rRNA Genes
Vitamin D
Carrier Proteins
Proteins
Adenosine Triphosphate
RNA

ASJC Scopus subject areas

  • Biochemistry

Cite this

The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. / Cavellán, Erica; Asp, Patrik; Percipalle, Piergiorgio; Farrants, Ann Kristin Östlund.

In: Journal of Biological Chemistry, Vol. 281, No. 24, 16.06.2006, p. 16264-16271.

Research output: Contribution to journalArticle

Cavellán, Erica ; Asp, Patrik ; Percipalle, Piergiorgio ; Farrants, Ann Kristin Östlund. / The WSTF-SNF2h chromatin remodeling complex interacts with several nuclear proteins in transcription. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 24. pp. 16264-16271.
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