The twin spot generator for differential Drosophila lineage analysis

Ruth Griffin, Anne Sustar, Marianne Bonvin, Richard Binari, Alberto del Valle Rodriguez, Amber M. Hohl, Jack R. Bateman, Christians Villalta, Elleard Heffern, Didier Grunwald, Chris Bakal, Claude Desplan, Gerold Schubiger, C. Ting Wu, Norbert Perrimon

Research output: Contribution to journalArticle

Abstract

In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

Original languageEnglish (US)
Pages (from-to)600-602
Number of pages3
JournalNature methods
Volume6
Issue number8
DOIs
StatePublished - Jul 28 2009

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Griffin, R., Sustar, A., Bonvin, M., Binari, R., del Valle Rodriguez, A., Hohl, A. M., Bateman, J. R., Villalta, C., Heffern, E., Grunwald, D., Bakal, C., Desplan, C., Schubiger, G., Wu, C. T., & Perrimon, N. (2009). The twin spot generator for differential Drosophila lineage analysis. Nature methods, 6(8), 600-602. https://doi.org/10.1038/nmeth.1349