The transcription/migration interface in heart precursors of Ciona intestinalis

Lionel Christiaen, Brad Davidson, Takeshi Kawashima, Weston Powell, Hector Nolla, Karen Vranizan, Michael Levine

Research output: Contribution to journalArticle

Abstract

Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.

Original languageEnglish (US)
Pages (from-to)1349-1352
Number of pages4
JournalScience
Volume320
Issue number5881
DOIs
StatePublished - Jun 6 2008

Fingerprint

Ciona intestinalis
Gene Regulatory Networks
Cell Surface Extensions
Forkhead Transcription Factors
Urochordata
Cell Polarity
Behavior Control
Fibroblast Growth Factors
Guanosine
Morphogenesis
Embryonic Development
Cell Movement
Up-Regulation
Membranes
Genes

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Christiaen, L., Davidson, B., Kawashima, T., Powell, W., Nolla, H., Vranizan, K., & Levine, M. (2008). The transcription/migration interface in heart precursors of Ciona intestinalis. Science, 320(5881), 1349-1352. https://doi.org/10.1126/science.1158170

The transcription/migration interface in heart precursors of Ciona intestinalis. / Christiaen, Lionel; Davidson, Brad; Kawashima, Takeshi; Powell, Weston; Nolla, Hector; Vranizan, Karen; Levine, Michael.

In: Science, Vol. 320, No. 5881, 06.06.2008, p. 1349-1352.

Research output: Contribution to journalArticle

Christiaen, L, Davidson, B, Kawashima, T, Powell, W, Nolla, H, Vranizan, K & Levine, M 2008, 'The transcription/migration interface in heart precursors of Ciona intestinalis', Science, vol. 320, no. 5881, pp. 1349-1352. https://doi.org/10.1126/science.1158170
Christiaen L, Davidson B, Kawashima T, Powell W, Nolla H, Vranizan K et al. The transcription/migration interface in heart precursors of Ciona intestinalis. Science. 2008 Jun 6;320(5881):1349-1352. https://doi.org/10.1126/science.1158170
Christiaen, Lionel ; Davidson, Brad ; Kawashima, Takeshi ; Powell, Weston ; Nolla, Hector ; Vranizan, Karen ; Levine, Michael. / The transcription/migration interface in heart precursors of Ciona intestinalis. In: Science. 2008 ; Vol. 320, No. 5881. pp. 1349-1352.
@article{e846b58992cd46abb09761fbdf1ba322,
title = "The transcription/migration interface in heart precursors of Ciona intestinalis",
abstract = "Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.",
author = "Lionel Christiaen and Brad Davidson and Takeshi Kawashima and Weston Powell and Hector Nolla and Karen Vranizan and Michael Levine",
year = "2008",
month = "6",
day = "6",
doi = "10.1126/science.1158170",
language = "English (US)",
volume = "320",
pages = "1349--1352",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "5881",

}

TY - JOUR

T1 - The transcription/migration interface in heart precursors of Ciona intestinalis

AU - Christiaen, Lionel

AU - Davidson, Brad

AU - Kawashima, Takeshi

AU - Powell, Weston

AU - Nolla, Hector

AU - Vranizan, Karen

AU - Levine, Michael

PY - 2008/6/6

Y1 - 2008/6/6

N2 - Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.

AB - Gene regulatory networks direct the progressive determination of cell fate during embryogenesis, but how they control cell behavior during morphogenesis remains largely elusive. Cell sorting, microarrays, and targeted molecular manipulations were used to analyze cardiac cell migration in the ascidian Ciona intestinalis. The heart network regulates genes involved in most cellular activities required for migration, including adhesion, cell polarity, and membrane protrusions. We demonstrated that fibroblast growth factor signaling and the forkhead transcription factor FoxF directly upregulate the small guanosine triphosphatase RhoDF, which synergizes with Cdc42 to contribute to the protrusive activity of migrating cells. Moreover, RhoDF induces membrane protrusions independently of other cellular activities required for migration. We propose that transcription regulation of specific effector genes determines the coordinated deployment of discrete cellular modules underlying migration.

UR - http://www.scopus.com/inward/record.url?scp=45549105130&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=45549105130&partnerID=8YFLogxK

U2 - 10.1126/science.1158170

DO - 10.1126/science.1158170

M3 - Article

C2 - 18535245

AN - SCOPUS:45549105130

VL - 320

SP - 1349

EP - 1352

JO - Science

JF - Science

SN - 0036-8075

IS - 5881

ER -