The transcription factor Sox9 is required for cranial neural crest development in Xenopus

Rebecca F. Spokony, Yoichiro Aoki, Natasha Saint-Germain, Emily Magner-Fink, Jean-Pierre Saint-Jeannet

Research output: Contribution to journalArticle

Abstract

The SOX family of transcription factors has been implicated in cell fate specification during embryogenesis. One member of this family, Sox9, has been shown to regulate both chondrogenesis and sex determination in the mouse embryo. Heterozygous mutations in Sox9 result in Campomelic Dysplasia (CD), a lethal human disorder characterized by autosomal XY sex reversal, severe skeletal malformations and several craniofacial defects. Sox9 is also expressed in neural crest progenitors but very little is known about the function of Sox9 in the neural crest. We have cloned the Xenopus homolog of the Sox9 gene. It is expressed maternally and accumulates shortly after gastrulation at the lateral edges of the neural plate, in the neural crest-forming region. As development proceeds, Sox9 expression persists in migrating cranial crest cells as they populate the pharyngeal arches. Depletion of Sox9 protein in developing embryos, using morpholino antisense oligos, causes a dramatic loss of neural crest progenitors and an expansion of the neural plate. Later during embryogenesis, morpholino-treated embryos have a specific loss or reduction of neural crest-derived skeletal elements, mimicking one aspect of the craniofacial defects observed in CD patients. We propose that Sox9 is an essential component of the regulatory pathway that leads to cranial neural crest formation.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalDevelopment
Volume129
Issue number2
StatePublished - 2002

Fingerprint

Neural Crest
Xenopus
Transcription Factors
Campomelic Dysplasia
Neural Plate
Morpholinos
Embryonic Structures
SOX Transcription Factors
Embryonic Development
Branchial Region
Chondrogenesis
Gastrulation
Mutation
Genes
Proteins

Keywords

  • Craniofacial skeleton
  • Morpholino antisense
  • Neural crest
  • Sox9
  • Xenopus

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Spokony, R. F., Aoki, Y., Saint-Germain, N., Magner-Fink, E., & Saint-Jeannet, J-P. (2002). The transcription factor Sox9 is required for cranial neural crest development in Xenopus. Development, 129(2), 421-432.

The transcription factor Sox9 is required for cranial neural crest development in Xenopus. / Spokony, Rebecca F.; Aoki, Yoichiro; Saint-Germain, Natasha; Magner-Fink, Emily; Saint-Jeannet, Jean-Pierre.

In: Development, Vol. 129, No. 2, 2002, p. 421-432.

Research output: Contribution to journalArticle

Spokony, RF, Aoki, Y, Saint-Germain, N, Magner-Fink, E & Saint-Jeannet, J-P 2002, 'The transcription factor Sox9 is required for cranial neural crest development in Xenopus', Development, vol. 129, no. 2, pp. 421-432.
Spokony, Rebecca F. ; Aoki, Yoichiro ; Saint-Germain, Natasha ; Magner-Fink, Emily ; Saint-Jeannet, Jean-Pierre. / The transcription factor Sox9 is required for cranial neural crest development in Xenopus. In: Development. 2002 ; Vol. 129, No. 2. pp. 421-432.
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