The transcription factor Mef2 is required for normal circadian behavior in Drosophila

Florence J. Blanchard, Ben Collins, Shawn A. Cyran, Daniel H. Hancock, Michael V. Taylor, Justin Blau

Research output: Contribution to journalArticle

Abstract

The transcription factor Mef2 has well established roles in muscle development in Drosophila and in the differentiation of many cell types in mammals, including neurons. Here, we describe a role for Mef2 in the Drosophila pacemaker neurons that regulate circadian behavioral rhythms. We found that Mef2 is normally produced in all adult clock neurons and that Mef2 overexpression in clock neurons leads to long period and complex rhythms of adult locomotor behavior. Knocking down Mef2 expression via RNAi or expressing a repressor form of Mef2 caused flies to lose circadian behavioral rhythms. These behavioral changes are correlated with altered molecular clocks in pacemaker neurons: Mef2 overexpression causes the oscillations in individual pacemaker neurons to become desynchronized, while Mef2 knockdown strongly dampens molecular rhythms. Thus, a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms.

Original languageEnglish (US)
Pages (from-to)5855-5865
Number of pages11
JournalJournal of Neuroscience
Volume30
Issue number17
DOIs
StatePublished - Apr 28 2010

Fingerprint

Drosophila
Transcription Factors
Neurons
Circadian Rhythm
Muscle Development
RNA Interference
Diptera
Cell Differentiation
Mammals

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

The transcription factor Mef2 is required for normal circadian behavior in Drosophila. / Blanchard, Florence J.; Collins, Ben; Cyran, Shawn A.; Hancock, Daniel H.; Taylor, Michael V.; Blau, Justin.

In: Journal of Neuroscience, Vol. 30, No. 17, 28.04.2010, p. 5855-5865.

Research output: Contribution to journalArticle

Blanchard, Florence J. ; Collins, Ben ; Cyran, Shawn A. ; Hancock, Daniel H. ; Taylor, Michael V. ; Blau, Justin. / The transcription factor Mef2 is required for normal circadian behavior in Drosophila. In: Journal of Neuroscience. 2010 ; Vol. 30, No. 17. pp. 5855-5865.
@article{276aeb2224ea4b83a81766a58a1f6e26,
title = "The transcription factor Mef2 is required for normal circadian behavior in Drosophila",
abstract = "The transcription factor Mef2 has well established roles in muscle development in Drosophila and in the differentiation of many cell types in mammals, including neurons. Here, we describe a role for Mef2 in the Drosophila pacemaker neurons that regulate circadian behavioral rhythms. We found that Mef2 is normally produced in all adult clock neurons and that Mef2 overexpression in clock neurons leads to long period and complex rhythms of adult locomotor behavior. Knocking down Mef2 expression via RNAi or expressing a repressor form of Mef2 caused flies to lose circadian behavioral rhythms. These behavioral changes are correlated with altered molecular clocks in pacemaker neurons: Mef2 overexpression causes the oscillations in individual pacemaker neurons to become desynchronized, while Mef2 knockdown strongly dampens molecular rhythms. Thus, a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms.",
author = "Blanchard, {Florence J.} and Ben Collins and Cyran, {Shawn A.} and Hancock, {Daniel H.} and Taylor, {Michael V.} and Justin Blau",
year = "2010",
month = "4",
day = "28",
doi = "10.1523/JNEUROSCI.2688-09.2010",
language = "English (US)",
volume = "30",
pages = "5855--5865",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "17",

}

TY - JOUR

T1 - The transcription factor Mef2 is required for normal circadian behavior in Drosophila

AU - Blanchard, Florence J.

AU - Collins, Ben

AU - Cyran, Shawn A.

AU - Hancock, Daniel H.

AU - Taylor, Michael V.

AU - Blau, Justin

PY - 2010/4/28

Y1 - 2010/4/28

N2 - The transcription factor Mef2 has well established roles in muscle development in Drosophila and in the differentiation of many cell types in mammals, including neurons. Here, we describe a role for Mef2 in the Drosophila pacemaker neurons that regulate circadian behavioral rhythms. We found that Mef2 is normally produced in all adult clock neurons and that Mef2 overexpression in clock neurons leads to long period and complex rhythms of adult locomotor behavior. Knocking down Mef2 expression via RNAi or expressing a repressor form of Mef2 caused flies to lose circadian behavioral rhythms. These behavioral changes are correlated with altered molecular clocks in pacemaker neurons: Mef2 overexpression causes the oscillations in individual pacemaker neurons to become desynchronized, while Mef2 knockdown strongly dampens molecular rhythms. Thus, a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms.

AB - The transcription factor Mef2 has well established roles in muscle development in Drosophila and in the differentiation of many cell types in mammals, including neurons. Here, we describe a role for Mef2 in the Drosophila pacemaker neurons that regulate circadian behavioral rhythms. We found that Mef2 is normally produced in all adult clock neurons and that Mef2 overexpression in clock neurons leads to long period and complex rhythms of adult locomotor behavior. Knocking down Mef2 expression via RNAi or expressing a repressor form of Mef2 caused flies to lose circadian behavioral rhythms. These behavioral changes are correlated with altered molecular clocks in pacemaker neurons: Mef2 overexpression causes the oscillations in individual pacemaker neurons to become desynchronized, while Mef2 knockdown strongly dampens molecular rhythms. Thus, a normal level of Mef2 activity is required in clock neurons to maintain robust and accurate circadian behavioral rhythms.

UR - http://www.scopus.com/inward/record.url?scp=77951654682&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77951654682&partnerID=8YFLogxK

U2 - 10.1523/JNEUROSCI.2688-09.2010

DO - 10.1523/JNEUROSCI.2688-09.2010

M3 - Article

C2 - 20427646

AN - SCOPUS:77951654682

VL - 30

SP - 5855

EP - 5865

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 17

ER -