The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis

Nanaho Fukuda, Tomoyuki Fukuda, John Sinnamon, Abrahan Hernandez-Hernandez, Manizheh Izadi, Chandrasekhar S. Raju, Kevin Czaplinski, Piergiorgio Percipalle

    Research output: Contribution to journalArticle

    Abstract

    During spermatogenesis, mRNA localization and translation are believed to be regulated in a stage-specific manner. We report here that the Protamine2 (Prm2) mRNA transits through chromatoid bodies of round spermatids and localizes to cytosol of elongating spermatids for translation. The transacting factor CBF-A, also termed Hnrnpab, contributes to temporal regulation of Prm2 translation. We found that CBF-A co-localizes with the Prm2 mRNA during spermatogenesis, directly binding to the A2RE/RTS element in the 3′ UTR. Although both p37 and p42 CBF-A isoforms interacted with RTS, they associated with translationally repressed and de-repressed Prm2 mRNA, respectively. Only p42 was found to interact with the 5′cap complex, and to co-sediment with the Prm2 mRNA in polysomes. In CBF-A knockout mice, expression of protamine 2 (PRM2) was reduced and the Prm2 mRNA was prematurely translated in a subset of elongating spermatids. Moreover, a high percentage of sperm from the CBF-A knockout mouse showed abnormal DNA morphology. We suggest that CBF-A plays an important role in spermatogenesis by regulating stage-specific translation of testicular mRNAs.

    Original languageEnglish (US)
    Article numbere1003858
    JournalPLoS Genetics
    Volume9
    Issue number10
    DOIs
    StatePublished - Oct 1 2013

    Fingerprint

    protamines
    Spermatogenesis
    spermatogenesis
    sperm
    translation (genetics)
    spermatids
    Spermatids
    DNA
    Messenger RNA
    mice
    sediment
    Protein Biosynthesis
    Knockout Mice
    polyribosomes
    3' untranslated regions
    cytosol
    Polyribosomes
    3' Untranslated Regions
    Cytosol
    spermatozoa

    ASJC Scopus subject areas

    • Genetics
    • Molecular Biology
    • Ecology, Evolution, Behavior and Systematics
    • Cancer Research
    • Genetics(clinical)

    Cite this

    The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis. / Fukuda, Nanaho; Fukuda, Tomoyuki; Sinnamon, John; Hernandez-Hernandez, Abrahan; Izadi, Manizheh; Raju, Chandrasekhar S.; Czaplinski, Kevin; Percipalle, Piergiorgio.

    In: PLoS Genetics, Vol. 9, No. 10, e1003858, 01.10.2013.

    Research output: Contribution to journalArticle

    Fukuda, N, Fukuda, T, Sinnamon, J, Hernandez-Hernandez, A, Izadi, M, Raju, CS, Czaplinski, K & Percipalle, P 2013, 'The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis', PLoS Genetics, vol. 9, no. 10, e1003858. https://doi.org/10.1371/journal.pgen.1003858
    Fukuda, Nanaho ; Fukuda, Tomoyuki ; Sinnamon, John ; Hernandez-Hernandez, Abrahan ; Izadi, Manizheh ; Raju, Chandrasekhar S. ; Czaplinski, Kevin ; Percipalle, Piergiorgio. / The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis. In: PLoS Genetics. 2013 ; Vol. 9, No. 10.
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