The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis

Nanaho Fukuda, Tomoyuki Fukuda, John Sinnamon, Abrahan Hernandez-Hernandez, Manizheh Izadi, Chandrasekhar S. Raju, Kevin Czaplinski, Piergiorgio Percipalle

Research output: Contribution to journalArticle

Abstract

During spermatogenesis, mRNA localization and translation are believed to be regulated in a stage-specific manner. We report here that the Protamine2 (Prm2) mRNA transits through chromatoid bodies of round spermatids and localizes to cytosol of elongating spermatids for translation. The transacting factor CBF-A, also termed Hnrnpab, contributes to temporal regulation of Prm2 translation. We found that CBF-A co-localizes with the Prm2 mRNA during spermatogenesis, directly binding to the A2RE/RTS element in the 3′ UTR. Although both p37 and p42 CBF-A isoforms interacted with RTS, they associated with translationally repressed and de-repressed Prm2 mRNA, respectively. Only p42 was found to interact with the 5′cap complex, and to co-sediment with the Prm2 mRNA in polysomes. In CBF-A knockout mice, expression of protamine 2 (PRM2) was reduced and the Prm2 mRNA was prematurely translated in a subset of elongating spermatids. Moreover, a high percentage of sperm from the CBF-A knockout mouse showed abnormal DNA morphology. We suggest that CBF-A plays an important role in spermatogenesis by regulating stage-specific translation of testicular mRNAs.

Original languageEnglish (US)
Article numbere1003858
JournalPLoS Genetics
Volume9
Issue number10
DOIs
StatePublished - Oct 1 2013

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protamines
Spermatogenesis
spermatogenesis
sperm
translation (genetics)
spermatids
Spermatids
DNA
Messenger RNA
mice
sediment
Protein Biosynthesis
Knockout Mice
polyribosomes
3' untranslated regions
cytosol
Polyribosomes
3' Untranslated Regions
Cytosol
spermatozoa

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Cancer Research
  • Genetics(clinical)

Cite this

The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis. / Fukuda, Nanaho; Fukuda, Tomoyuki; Sinnamon, John; Hernandez-Hernandez, Abrahan; Izadi, Manizheh; Raju, Chandrasekhar S.; Czaplinski, Kevin; Percipalle, Piergiorgio.

In: PLoS Genetics, Vol. 9, No. 10, e1003858, 01.10.2013.

Research output: Contribution to journalArticle

Fukuda, Nanaho ; Fukuda, Tomoyuki ; Sinnamon, John ; Hernandez-Hernandez, Abrahan ; Izadi, Manizheh ; Raju, Chandrasekhar S. ; Czaplinski, Kevin ; Percipalle, Piergiorgio. / The Transacting Factor CBF-A/Hnrnpab Binds to the A2RE/RTS Element of Protamine 2 mRNA and Contributes to Its Translational Regulation during Mouse Spermatogenesis. In: PLoS Genetics. 2013 ; Vol. 9, No. 10.
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