The Tnt1 retrotransposon escapes silencing in tobacco, its natural host

Inmaculada Hernández-Pinzón, Marta Cifuentes, Elizabeth Henaff, Néstor Santiago, M. Lluïsa Espinás, Josep M. Casacuberta

Research output: Contribution to journalArticle

Abstract

Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host.

Original languageEnglish (US)
Article numbere33816
JournalPLoS One
Volume7
Issue number3
DOIs
StatePublished - Mar 30 2012

Fingerprint

Retroelements
Tobacco
retrotransposons
tobacco
Genes
promoter regions
Plant Genome
Plant Viruses
Mutagenesis
genome
plant viruses
Gene Silencing
gene silencing
DNA methylation
DNA Methylation
Transgenes
Viruses
mutagenesis
transgenes
Genome

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Hernández-Pinzón, I., Cifuentes, M., Henaff, E., Santiago, N., Espinás, M. L., & Casacuberta, J. M. (2012). The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. PLoS One, 7(3), [e33816]. https://doi.org/10.1371/journal.pone.0033816

The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. / Hernández-Pinzón, Inmaculada; Cifuentes, Marta; Henaff, Elizabeth; Santiago, Néstor; Espinás, M. Lluïsa; Casacuberta, Josep M.

In: PLoS One, Vol. 7, No. 3, e33816, 30.03.2012.

Research output: Contribution to journalArticle

Hernández-Pinzón, I, Cifuentes, M, Henaff, E, Santiago, N, Espinás, ML & Casacuberta, JM 2012, 'The Tnt1 retrotransposon escapes silencing in tobacco, its natural host', PLoS One, vol. 7, no. 3, e33816. https://doi.org/10.1371/journal.pone.0033816
Hernández-Pinzón I, Cifuentes M, Henaff E, Santiago N, Espinás ML, Casacuberta JM. The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. PLoS One. 2012 Mar 30;7(3). e33816. https://doi.org/10.1371/journal.pone.0033816
Hernández-Pinzón, Inmaculada ; Cifuentes, Marta ; Henaff, Elizabeth ; Santiago, Néstor ; Espinás, M. Lluïsa ; Casacuberta, Josep M. / The Tnt1 retrotransposon escapes silencing in tobacco, its natural host. In: PLoS One. 2012 ; Vol. 7, No. 3.
@article{81de778231e34df1a4ce6f429fe002b5,
title = "The Tnt1 retrotransposon escapes silencing in tobacco, its natural host",
abstract = "Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host.",
author = "Inmaculada Hern{\'a}ndez-Pinz{\'o}n and Marta Cifuentes and Elizabeth Henaff and N{\'e}stor Santiago and Espin{\'a}s, {M. Llu{\"i}sa} and Casacuberta, {Josep M.}",
year = "2012",
month = "3",
day = "30",
doi = "10.1371/journal.pone.0033816",
language = "English (US)",
volume = "7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

TY - JOUR

T1 - The Tnt1 retrotransposon escapes silencing in tobacco, its natural host

AU - Hernández-Pinzón, Inmaculada

AU - Cifuentes, Marta

AU - Henaff, Elizabeth

AU - Santiago, Néstor

AU - Espinás, M. Lluïsa

AU - Casacuberta, Josep M.

PY - 2012/3/30

Y1 - 2012/3/30

N2 - Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host.

AB - Retrotransposons' high capacity for mutagenesis is a threat that genomes need to control tightly. Transcriptional gene silencing is a general and highly effective control of retrotransposon expression. Yet, some retrotransposons manage to transpose and proliferate in plant genomes, suggesting that, as shown for plant viruses, retrotransposons can escape silencing. However no evidence of retrotransposon silencing escape has been reported. Here we analyze the silencing control of the tobacco Tnt1 retrotransposon and report that even though constructs driven by the Tnt1 promoter become silenced when stably integrated in tobacco, the endogenous Tnt1 elements remain active. Silencing of Tnt1-containing transgenes correlates with high DNA methylation and the inability to incorporate H2A.Z into their promoters, whereas the endogenous Tnt1 elements remain partially methylated at asymmetrical positions and incorporate H2A.Z upon induction. Our results show that the promoter of Tnt1 is a target of silencing in tobacco, but also that endogenous Tnt1 elements can escape this control and be expressed in their natural host.

UR - http://www.scopus.com/inward/record.url?scp=84859127324&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84859127324&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0033816

DO - 10.1371/journal.pone.0033816

M3 - Article

VL - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 3

M1 - e33816

ER -